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Plasma concentrations of ir-inhibin, inhibin A, inhibin pro-αC, FSH, and estradiol-17β during estrous cycle in mares and their relationship with follicular growth

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Abstract

The relationship among plasma levels of immunoreactive (ir)-inhibin, inhibin A, inhibin pro-αC, FSH, estradiol-17β and follicular growth were investigated during the normal estrous cycle in mares. Seven mares were used for two successive normal estrous cycles. Follicular diameters and ovulation were obtained by transrectal ultrasonography, and blood samples were collected daily by jugular venipuncture for hormonal assay. The results showed that inhibin A was inversely correlated (r=−0.59, p<0.0001) with FSH indicating its contribution to negative feedback control of FSH secretions from the pituitary gland. Estradiol-17β increased during the follicular phase reaching a peak (37.9±3.8 pg/mL) 2 d before ovulation. Estradiol-17β was positively correlated (r=0.78, p<0.0001) with inhibin A. The high levels of inhibin A and estradiol-17β were associated with the growth of the preovulatory dominant follicle and inversely correlated with FSH suggesting that both hormones are products of the large dominant follicles and were responsible for the decline in FSH secretion during the follicular phase of estrous cycle. In conclusion, an inverse relationship between inhibin A and FSH was clearly demonstrated indicating that inhibin A has a key role in the negative feedback control of FSH from the pituitary gland. In addition, inhibin A and estradiol-17β secretions were associated with the growth of the preovulatory dominant follicle and were positively correlated.

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References

  1. Knight, P. G. (1996). Front. Neuroendocrinol. 17, 476–509.

    Article  PubMed  CAS  Google Scholar 

  2. Vale, W. W., Hseuh, A., Rivier, C., and Yu, J. (1990). In: Peptide growth factors and their receptors: handbook of experimental physiology. (Sporn, M. A. and Roberts, A. B., eds.). Springer-Verlag: Berlin, pp. 211–248.

    Google Scholar 

  3. Mason, A. J., Farnworth, P. G., and Sullivan, J. (1996). Mol. Endocrinol. 10, 1055–1065.

    Article  PubMed  CAS  Google Scholar 

  4. Schneyer, A. L., Fujiwara, T., Fox, J., et al. (2000). J. Clin. Endocrinol. Metab. 85, 3319–3330.

    Article  PubMed  CAS  Google Scholar 

  5. Welt, C. K., Smith, Z. A., Pauler, D. K., and Hall, J. E. (2001). J. Clin. Endocrinol. Metab. 86, 2531–2537.

    Article  PubMed  CAS  Google Scholar 

  6. Beg, M. A., Bergfelt, D. R., Kot, K., and Ginther, O. J. (2002). Biol. Reprod. 66, 120–126.

    Article  PubMed  CAS  Google Scholar 

  7. Meunier, H., Cajander, S. B., Roberts, V. J., et al. (1988). Mol. Endocrinol. 2, 1352–1363.

    Article  PubMed  CAS  Google Scholar 

  8. Piquette, G. N., Kenney, R. M., Sertich, P. L., Yamato, M., and Hsuei, A. J. W. (1990). Biol. Reprod. 43, 1050–1057.

    Article  PubMed  CAS  Google Scholar 

  9. Taya, K., Kaneko, H., Watanabe, G., and Sasamoto, S. (1991). J. Reprod. Fertil. (Suppl.) 43, 151–162.

    CAS  Google Scholar 

  10. Taya, K., Kaneko, H., Takedomi, T., Kishi, H., and Watanabe, G. (1996). Anim. Reprod. Sci. 42, 563–570.

    Article  CAS  Google Scholar 

  11. Engelhardt, H., Smith, K. B., McNeilly, A. S., and Baird, D. T. (1993). Biol. Reprod. 49, 281–294.

    Article  PubMed  CAS  Google Scholar 

  12. Bergfelt, D. R., Mann, B. G., Schwartz, N. B., and Ginther, O. J. (1991). Equine Vet. J. 11, 319–322.

    Google Scholar 

  13. Roser, J. F., McCue, P. M., and Hoye, E. (1994). Domest. Anim. Endocrinol. 11, 87–100.

    Article  PubMed  CAS  Google Scholar 

  14. Nagamine, N., Nambo, Y., Nagata, S., et al. (1998). Biol. Reprod. 59, 1392–1398.

    Article  PubMed  CAS  Google Scholar 

  15. McNeilly, A. S., Brooks, A. N., Baxter, G., and Webb, R. (1994). Front. Endocrinol. 3, 261–269.

    CAS  Google Scholar 

  16. Donadeu, F. X. and Ginther, O. J. (2002). Biol. Reprod. 66, 1111–1118.

    Article  PubMed  CAS  Google Scholar 

  17. Watson, E. D., Thomassen, R., Steele, M., et al. (2002). Anim. Reprod. Sci. 74, 55–67.

    Article  PubMed  CAS  Google Scholar 

  18. Nagaoka, K., Nambo, Y., Nagamine, N., et al. (1999). Am. J. Physiol. 277, E870-E875.

    PubMed  CAS  Google Scholar 

  19. Tanaka, Y., Nagamine, N., Nagata, S., et al. (2000). J. Reprod. Fertil. (Suppl.) 56, 239–245.

    Article  Google Scholar 

  20. Nambo, Y., Nagaoka, K., Tanaka, Y., et al. (2002). Theriogenology 57, 1707–1717.

    Article  PubMed  CAS  Google Scholar 

  21. Bergfelt, D. R. and Ginther, O. J. (1993). Theriogenology 39, 781–786.

    Article  PubMed  CAS  Google Scholar 

  22. Nambo, Y., Kaneko, H., Nagata, S., et al. (1998). Theriogenology 50, 545–557.

    Article  PubMed  CAS  Google Scholar 

  23. Miller, K. F., Wesson, J. A., and Ginther, O. J. (1979). Biol. Reprod. 21, 867–872.

    Article  PubMed  CAS  Google Scholar 

  24. McCue, P. M., Canney, N. J., Hughes, J. P., Rivier, J., Vale, W., and Lasley, B. L. (1992). Theriogenology 38, 823–831.

    Article  PubMed  CAS  Google Scholar 

  25. Sharp, D. C., Grubaugh, W. R., Weithenauer, J., Davis, S. D., and Wilcox, C. J. (1991). Biol. Reprod. 44, 983–990.

    Article  PubMed  CAS  Google Scholar 

  26. Gastal, E. L., Gastal, M. O., and Ginther, O. J. (1999). Biol. Reprod. 61, 724–730.

    Article  PubMed  CAS  Google Scholar 

  27. Gastal, E. L., Gastal, M. O., Wiltbank, M. C., and Ginther, O. J. (1999). Biol. Reprod. 61, 31–39.

    Article  PubMed  CAS  Google Scholar 

  28. Donadeu, F. X. and Ginther, O. J. (2001). Reproduction 121, 897–903.

    Article  PubMed  CAS  Google Scholar 

  29. Bleach, E. C. L., Glencross, R. G., Feist, S. A., Groome, N. P., and Knight, P. G. (2001). Biol. Reprod. 64, 743–752.

    Article  PubMed  CAS  Google Scholar 

  30. Hamada, T., Watanabe, G., Kokuho, T., et al. (1989). J. Endocrinol. 122, 697–704.

    PubMed  CAS  Google Scholar 

  31. Taya, K., Watanabe, G., and Sasamoto, S. (1985). Jpn. J. Anim. Reprod. 31, 186–197.

    CAS  Google Scholar 

  32. Nagata, S., Kondou, M., Kaneko, H., et al. (1996). J. Reprod. Dev. 42, j43-j49.

    CAS  Google Scholar 

  33. Groome, N. P., Illingworth, P. J., O’Brien, M., Priddle, J., Weaver, K., and McNeilly, A. S. (1995). J. Clin. Endocrinol. Metab. 80, 2926–2932.

    Article  PubMed  CAS  Google Scholar 

  34. Souza, C. J. H., Campbell, B. K., Webb, R., and Baird, D. T. (1997). Endocrinol. 138, 5333–5340.

    Article  CAS  Google Scholar 

  35. Knight, P. G., Feist, S. A., Tannetta, D. S., et al. (1998). J. Reprod. Fertil. 113, 159–166.

    Article  PubMed  CAS  Google Scholar 

  36. Tanaka, Y., Taniyama, H., Tsunoda, N., et al. (2002). J. Androl. 23, 229–236.

    PubMed  CAS  Google Scholar 

  37. Medan, M. S., Watanabe, G., Sasaki, K., Sharawy, S., Groome, N. P., and Taya, K. (2003). Biol. Reprod. 69, 57–63.

    Article  PubMed  CAS  Google Scholar 

  38. SAS. (1987). Statistics, version 6.11, Cary, NC: SAS Institute Inc.

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Authors and Affiliations

  1. Labortory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan

    Mohamed S. Medan, Gen Watanabe & Kazuyoshi Taya DVM PhD

  2. Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt

    Mohamed S. Medan

  3. Hidaka Training Research Center, Japan Racing Association, 057-171, Hokkaido, Japan

    Yasuo Nambo

  4. Northern Farm, 059-4132, Hokkaido, Japan

    Natsuko Nagamine

  5. Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan

    Hiromi Shinbo, Gen Watanabe & Kazuyoshi Taya DVM PhD

  6. School of Biological and Molecular Sciences, Oxford Brookes University, OX3 OBP, Oxford, UK

    Nigel Groome

Authors
  1. Mohamed S. Medan
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  2. Yasuo Nambo
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  3. Natsuko Nagamine
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  4. Hiromi Shinbo
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  5. Gen Watanabe
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  6. Nigel Groome
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  7. Kazuyoshi Taya DVM PhD
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Correspondence to Kazuyoshi Taya DVM PhD.

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Medan, M.S., Nambo, Y., Nagamine, N. et al. Plasma concentrations of ir-inhibin, inhibin A, inhibin pro-αC, FSH, and estradiol-17β during estrous cycle in mares and their relationship with follicular growth. Endocr 25, 7–14 (2004). https://doi.org/10.1385/ENDO:25:1:07

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  • DOI: https://doi.org/10.1385/ENDO:25:1:07

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