Abstract
Hypoglycemia inhibits gonadotropin secretion in primates by an undefined mechanism. Some evidence suggests that hypoglycemia inhibits gonadotropin secretion independent of gonadotropin-releasing hormone (GnRH) inhibition. To this end, the effect of insulin-induced hypoglycemia on the luteinizing hormone (LH) and follicle-stimulating hormone (FSH) response to graded doses of GnRH (25, 75, and 250 ng/kg) administered at 120-min intervals was determined in rhesus monkeys. A crossover design was employed such that each animal received GnRH under both hypoglycemic and euglycemic conditions. Experiments were performed in the follicular phase. Gonadotroph responsiveness to GnRH was quantified by determining the change in area under the LH (ΔAULHC) and FSH (ΔAUFSHC) curves that occurred in the first 60 min following each GnRH pulse. There was no statistical difference in ΔAULHC between euglycemic and hypoglycemic animals at any GnRH dose (25 ng/kg: p=0.19; 75 ng/kg: p=0.41; 250 ng/kg: p=0.46). Similarly, changes in AUFSHC following GnRH administration were comparable in euglycemic and hypoglycemic animals (25 ng/kg: p=0.59; 75 ng/kg: p=0.90; 250 ng/kg: p=0.33). We conclude that hypoglycemia had no effect on gonadotroph responsiveness to GnRH. These results are consistent with the conclusion that hypoglycemia inhibits gonadotropin secretion by acting primarily at the level of the hypothalamus to reduce GnRH secretion rather than affecting pituitary responsiveness to GnRH.
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Catt, K. J., Loumaye, E., Wynn, P. C., et al. (1985). J. Steroid Biochem. 23, 677–689.
Pau, K. Y. and Spies, H. G. (1997). Chin. J. Physiol. 40, 181–196.
Martin, A. I., Lopez-Calderon, A., Tresguerres, J. A., Gonzalez-Quijano, M. I., and Cardinali, D. P. (1995). Neuroendocrinology 61, 173–179.
Heisler, L. E., Tumber, A. J., Reid, R. L., and Van Vugt, D. A. (1994). Neuroendocrinology 60, 297–304.
Rettori, V. and McCann, S. M. (1997). Mol. Psychiatry 2, 350–354.
Leonhardt, S., Shahab, M., Luft, H., Wuttke, W., and Jarry, H. (1999). J. Neuroendocrinol. 11, 613–619.
Xiao, E., Xia-Zhang, L., and Ferin, M. (2000). Neuroimmunomodulation 7, 6–15.
Bishop, W., Fawcett, C. P., Krulich, L., and McCann, S. M. (1972). Endocrinology 91, 643–656.
Zeballos, G. and McCann, S. M. (1977). Proc. Soc. Exp. Biol. Med. 154, 242–245.
Ching, M. C., Blank, M. S., Catt, K. J., Negro-Vilar, A., and Dufau, M. L. (1986). Soc. Neurosci. Abstr. 12, 1411.
Frawley, L. S., Dailey, R. A., Tindall, G. T., and Neill, J. D. (1981). Neuroendocrinology 32, 14–18.
Hwan, J. C. and Freeman, M. E. (1987). Endocrinology 121, 1099–1103.
Goubillon, M. L. and Thalabard, J. C. (1996). Neuroendocrinology 64, 49–56.
Clarke, I. J., Horton, R. J., and Doughton, B. W. (1990). Endocrinology 127, 1470–1476.
Chen, M. D., O’Byrne, K. T., Chiappini, S. E., Hotchkiss, J., and Knobil, E. (1992). Neuroendocrinology 56, 666–673.
Heisler, L. E., Pallotta, C. M., Reid, R. L., and Van Vugt, D. A. (1993). J. Clin. Endocrinol. Metab. 76, 1280–1285.
Van Vugt, D. A., Washburn, D. L., Farley, A. E., and Reid, R. L. (1997). Neuroendocrinology 65, 344–352.
Altman, D. G. (1991). Practical statistics for medical research. Chapman & Hall: London, UK.
Adam, C. L. and Findlay, P. A. (1998). J. Neuroendocrinol. 10, 777–783.
Van Vugt, D., Roy, B., Shridhar, S., and Reid, R. (1999). Conjoint Meeting of ASRM/CFAS, Toronto, Ontario, Canada.
Guillaume, V., Grino, M., Conte-Devolx, B., Boudouresque, F., and Oliver, C. (1989). Neuroendocrinology 49, 676–679.
Engler, D., Pham, T., Fullerton, M. J., Ooi, G., Funder, J. W., and Clarke, I. J. (1989). Neuroendocrinology 49, 367–381.
Caraty, A., Grino, M., Locatelli, A., et al. (1990). J. Clin. Invest. 85, 1716–1721.
Chen, M. D., Ordog, T., O’Byrne, K. T., Goldsmith, J. R., Connaughton, M. A., and Knobil, E. (1996). Endocrinology 137, 2012–2021.
Gindoff, P. R. and Ferin, M. (1987). Endocrinology 121, 837–842.
Barbarino, A., De Marinis, L., Tofani, A., et al. (1989). J. Clin. Endocrinol. Metab. 68, 523–528.
Williams, C. L., Nishihara, M., Thalabard, J. C., Grosser, P. M., Hotchkiss, J., and Knobil, E. (1990). Neuroendocrinology 52, 133–137.
Plotsky, P. M., Bruhn, T. O., and Vale, W. (1985). Endocrinology 117, 323–329.
Lado-Abeal, J., Clapper, J. A., and Norman, R. L. (2001). J. Neuroendocrinol. 13, 650–655.
Caffrey, M. H., Nett, T. M., and Kozlowski, G. P. (1978). Proc. Soc. Exp. Biol. Med. 159, 444–448.
Cheung, C. Y. (1983). Endocrinology 113, 632–638.
Ono, N., Samson, W. K., McDonald, J. K., et al. (1985). Proc. Natl. Acad. Sci. USA 82, 7787–7790.
Blumenfeld, Z., Kuhn, R. W., and Jaffe, R. B. (1986). Am. J. Obstet. Gynecol. 154, 606–612.
Harsoulis, P., Marshall, J. C., Kuku, S. F., Burke, C. W., London, D. R., and Fraser, T. R. (1973). BMJ 4, 326–329.
Mortimer, C. H., Besser, G. M., McNelly, A. S., Tunbridge, W. M. G., Gomez-Pan, A., and Hall, R. (1973). Clin. Endocrinol. 2, 317–326.
Seidell, J. C., Bjorntorp, P., Sjostrom, L., Kvist, H., and Sannerstedt, R. (1990). Metabolism 39, 897–901.
Oltmanns, K. M., Fruehwald-Schultes, B., Kern, W., Born, J., Fehm, H. L., and Achim, P. (2001). J. Clin. Endocrinol. Metab. 86(10), 4913–4919.
He, D., Funabashi, T., Sano, A., Uemura, T., Minaguchi, H., and Kimura, F. (1999). Brain Res. 820(1–2), 71–76.
Rodriguez, M., Arias, P., Refojo, D., Feleder, C., and Moguilevsky, J. (1990). J. Exp. Clin. Endocrinol. Diabetes 107(4), 257–261.
Adashi, E. Y., Hsueh, A. J., and Yen, S. S. (1981). Endocrinology 108(4), 1441–1449.
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Lujan, M.E., Krzemien, A.A. & Van Vugt, D.A. Hypoglycemia does not affect gonadotroph responsiveness to gonadotropin-releasing hormone in rhesus monkeys. Endocr 21, 109–114 (2003). https://doi.org/10.1385/ENDO:21:2:109
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DOI: https://doi.org/10.1385/ENDO:21:2:109