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Reciprocal regulation by estradiol 17-β of ezrin and cadherin-catenin complexes in pituitary GH3 cells

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Abstract

The antiestrogen, ICI 182780, and estradiol-17β (E2) regulate cadherin-mediated cell adhesion in pituitary GH3 cells. Using a cDNA expression array to screen for E2-regulated genes that are associated with the cytoskeleton, we observed that E2 stimulated ezrin gene expression and confirmed that ezrin gene expression is regulated pretranslationally by ICI 182780 versus E2. E2 increased ezrin protein levels in whole-cell lysates and in the cytoskeletal-associated, detergent-insoluble fraction. Confocal microscopy revealed that ezrin was associated with free apical membranes of E2-treated cells. E2 decreased N-cadherin and β-catenin levels and induced a redistribution of p120ctn to the cytoplasm. In GH3 transfectants overexpressing E-cadherin, E2 had no effect on adhesiveness or on E-cadherin and p120ctn distribution, but increased levels of active ezrin. Ezrin was concentrated at free and apical membranes. These studies provide the first demonstration of the regulation of ezrin by E2 and show that the ER signaling pathway coordinately regulates two cytoskeletal-associated protein complexes, with mutually exclusive cellular distributions, in a reciprocal manner. These findings indicate that E2 enriches the cell membrane with ezrin-membrane protein complexes by both increasing ezrin expression and by enlarging the relative area of nonadhesive membrane to which ezrin is targeted.

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References

  1. Cai, A., Bowers, R., Moore, J., and Hyde, J. (1998). Endocrinology 139, 24522458.

  2. Maurer, R., Kim, K., Day, R., and Notides, A. (1990). Prog. Clin. Biol. Res. 322, 159–169.

    PubMed  CAS  Google Scholar 

  3. Antakly, T., Pelletier, G., Zeytinoglu, F., and Labrie, F. (1980). J. Cell Biol. 86, 377–387.

    Article  PubMed  CAS  Google Scholar 

  4. Lloyd, R. (1983). Am. J. Pathol. 113, 198–206.

    PubMed  CAS  Google Scholar 

  5. Scheithauser, B., Sano, T., Kovacs, K., Young, W. J., Ryan, N., and Randall, R. (1990). Mayo Clin. Proc. 65, 461–473.

    Google Scholar 

  6. Stocker, H. and Hafen, E. (2000). Curr. Opin. Genet. Dev. 10, 529–535.

    Article  PubMed  CAS  Google Scholar 

  7. Spady, T., McComb, R., and Shull, J. (1999). Endocrine 11, 217–233.

    Article  PubMed  CAS  Google Scholar 

  8. Gonzalez, J., Elizondo, G., and Salvidar, D. (1988). Am. J. Med. 85, 217–220.

    Article  PubMed  CAS  Google Scholar 

  9. Lee, M., Zhu, Y., Sun, Z., Rhee, H., Jeromin, A., Roder, J., et al. (2000). Endocrinology 141, 3485–3492.

    Article  PubMed  CAS  Google Scholar 

  10. Heinrich, C. A., Lail-Trecker, M. R., Peluso, J. J., and White, B. A. (1999). Endocrine 10, 67–76.

    Article  PubMed  CAS  Google Scholar 

  11. Gusella, J. F., Ramesh, V., MacCollin, M., and Jacoby, L. B. (1999). Biochim. Biophys. Acta 1423, M29-M36.

    PubMed  CAS  Google Scholar 

  12. Bretscher, A., Reczek, D., and Berryman, M. (1997). J. Cell Sci. 110, 3011–3018.

    PubMed  CAS  Google Scholar 

  13. Tsukita, S., Yonemura, S., and Tsukita, S. (1997). Curr. Opin. Cell Biol. 9, 70–75.

    Article  PubMed  CAS  Google Scholar 

  14. Vaheri, A., Carpen, O., Heiska, L., Helander, T. S., Jaaskelainen, J., Majender-Nordenswan, P., et al. (1997). Curr. Opin. Cell Biol. 9, 659–666.

    Article  PubMed  CAS  Google Scholar 

  15. Bretscher, A. (1999). Curr. Opin. Cell Biol. 11, 109–116.

    Article  PubMed  CAS  Google Scholar 

  16. Mangeat, P., Roy, C., and Martin, M. (1999). Trends Cell Biol. 9, 187–192.

    Article  PubMed  CAS  Google Scholar 

  17. Tsukita, S. and Yonemura, S. (1999). J. Biol. Chem. 274, 34,507–34,510.

    Article  CAS  Google Scholar 

  18. Ediger, T., Kraus, W., Weinman, E., and Katzenellenbogen, B. (1999). Endocrinology 140, 2976–2982.

    Article  PubMed  CAS  Google Scholar 

  19. Theoharides, T. C., Wang, L., Pang, X., Letourneau, R., Culm, K. E., Basu, S., et al. (2000). J. Pharmacol. Exp. Ther. 294, 810–821.

    PubMed  CAS  Google Scholar 

  20. Gautreau, A., Poullet, P., Louvard, D., and Arpin, M. (1999). Proc. Natl. Acad. Sci. USA 96, 7300–7305.

    Article  PubMed  CAS  Google Scholar 

  21. Tran Quang, C., Gautreau, A., Arpin, M., and Treisman, R. (2000). EMBO J. 19, 4565–4576.

    Article  PubMed  CAS  Google Scholar 

  22. Lamb, R., Ozanne, B., Roy, C., McGarry, L., Stipp, C., Mangeat, P., and Jay, D. (1997). Curr. Biol. 7, 682–688.

    Article  PubMed  CAS  Google Scholar 

  23. DePasquale, J. A. (1999). Histochem. Cell Biol. 112, 341–350.

    Article  PubMed  CAS  Google Scholar 

  24. Potter, E., Bergwitz, C., and Brabant, G. (1999). Endocr. Rev. 20, 207–239.

    Article  PubMed  CAS  Google Scholar 

  25. Szelei, J., Soto, A., Geck, P., Desronvil, M., Prechtl, N., Weill, B., et al. (2000). J. Steroid Biochem. Mol. Biol. 72, 89–102.

    Article  PubMed  CAS  Google Scholar 

  26. Allen, D. L., Mitchner, N. A., Uveges, T. E., Nephew, K. P., Khan, S., and Ben-Jonathan, N. (1997). Endocrinology 138, 2128–2135.

    Article  PubMed  CAS  Google Scholar 

  27. Szijan, I., Parma, D. L., and Engel, N. I. (1992). Horm. Metab. Res. 24, 154–157.

    Article  PubMed  CAS  Google Scholar 

  28. Jooss, K. U. and Muller, R. (1995). Oncogene 10, 603–608.

    PubMed  CAS  Google Scholar 

  29. Heany, A., Horwitz, G., Wang, Z., Singson, R., and Melmed, S. (1999). Nat. Med. 5, 1317–1321.

    Article  CAS  Google Scholar 

  30. Chernavsky, A. C., Valerani, A. V., and Burdman, J. A. (1993). Neurol. Res. 15, 339–343.

    PubMed  CAS  Google Scholar 

  31. Takeuchi, K., Sato, N., Kasahara, H., Funayama, N., Nagafuchi, A., Yonemura, S., et al. (1994). J. Cell Biol. 125, 1371–1384.

    Article  PubMed  CAS  Google Scholar 

  32. Hiscox, S. and Jiang, W. (1999). J. Cell Sci. 112, 3081–3090.

    PubMed  CAS  Google Scholar 

  33. Mohler, P. J., Kreda, S. M., Boucher, R. C., Sudol, M., Stutts, M. J., and Milgram, S. L. (1999). J. Cell Biol. 147, 879–890.

    Article  PubMed  CAS  Google Scholar 

  34. Eastman, Q. and Grosschedl, R. (1999). Curr. Opin. Cell Biol. 11, 233–240.

    Article  PubMed  CAS  Google Scholar 

  35. Semba, S., Han, S.-Y., Ikeda, H., and Horii, A. (2001). Cancer 91, 42–48.

    Article  PubMed  CAS  Google Scholar 

  36. van Hengel, J., Vanhoenacker, P., Staes, K., and van Roy, F. (1999). Proc. Natl. Acad. Sci. USA 96, 7980–7985.

    Article  PubMed  Google Scholar 

  37. Daniel, J. M. and Reynolds, A. B. (1999). Mol. Cell. Biol. 19, 3614–3623.

    PubMed  CAS  Google Scholar 

  38. Grosheva, I., Shtutman, M., Elbaum, M., and Bershadsky, A. (2001). J. Cell. Sci. 114, 695–707.

    PubMed  CAS  Google Scholar 

  39. Anastasiadis, P., Moon, S., Thoreson, M., Mariner, D., Crawford, H., Zheng, Y., et al. (2000). Nat. Cell Biol. 2, 637–644.

    Article  PubMed  CAS  Google Scholar 

  40. Noren, N., Liu, B., Burridge, K., and Kreft, B. (2000). J. Cell. Biol. 150, 567–579.

    Article  PubMed  CAS  Google Scholar 

  41. Wick, W., Grimmel, C., Wild-Bode, C., Platten, M., Arpin, M., and Weller, M. (2001). J. Neurosci. 21, 3360–3368.

    PubMed  CAS  Google Scholar 

  42. Kaul, S., Mitsui, Y., Komatsu, Y., Reddel, R., and Wadhwa, R. (1996). Oncogene 13, 1231–1237.

    PubMed  CAS  Google Scholar 

  43. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., et al. (1989). Current protocols in molecular biology. John Wiley: New York.

    Google Scholar 

  44. Lee, M.-J., Thangada, S., Claffey, K., Ancelin, N., Liu, C., Kluk, M., et al. (1999). Cell 99, 301–312.

    Article  PubMed  CAS  Google Scholar 

  45. Ozawa, M. and Kemler, R. (1998). J. Biol. Chem. 273, 6166–6170.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Connecticut Health Center, Farmington, CT

    Ann Cowan

  2. Department of Physiology, University of Connecticut Health Center, MC 3505, 06030, Farmington, CT

    Perry M. Smith, C. Amanda Heinrich, Stacey Pappas, John J. Peluso &  Bruce A. White

  3. Department of Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, CT

    Ann Cowan

Authors
  1. Perry M. Smith
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  2. C. Amanda Heinrich
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  3. Stacey Pappas
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  4. John J. Peluso
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  5. Ann Cowan
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  6. Bruce A. White
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Correspondence to Bruce A. White.

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Smith, P.M., Heinrich, C.A., Pappas, S. et al. Reciprocal regulation by estradiol 17-β of ezrin and cadherin-catenin complexes in pituitary GH3 cells. Endocr 17, 219–228 (2002). https://doi.org/10.1385/ENDO:17:3:219

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  • DOI: https://doi.org/10.1385/ENDO:17:3:219

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