Skip to main content
Log in

Influence of polyelectrolyte multilayer films on the ICAM-1 expression of endothelial cells

  • Original Article
  • Published:
Cell Biochemistry and Biophysics Aims and scope Submit manuscript

Abstract

Recently, the use of polyelectrolyte films has been suggested as a new versatile technique of surface modification aimed at tissue engineering. In the present study, we evaluated the expression of intercellular adhesion molecule (ICAM)-1 of endothelial cells (ECs) seeded on two types of polyelectrolyte multilayer films either terminated by poly(d-lysine) (PDL) or poly(allylamine hydrochloride) (PAH). This work showed that chemical stimulations with tumor necrosis factor (TNF)-α induced the ICAM-1 expression of ECs differently depending largely on the film architecture employed. Compared with PAH-ending films, the PDL-ending ones upregulated the ICAM-1 expression of the ECs after a prolonged exposition to TNF-α, rendering this film type less favorable in tissue engineering. Cytochalasin D (an F-actin disrupting agent) showed the involvement of the cytoskeleton in the upregulation of ICAM-1 for cells deposited on films terminated by PDL. The PAH-ending films did not perturb the ICAM-1 expression of ECs and might thus enhance the seeding of ECs in vascular engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Seifalian, A. M., Tiwari, A., Hamilton, G., and Salacinski, H. J. (2002) Improving the clinical patency of prosthetic vascular and coronary bypass grafts: the role of seeding and tissue engineering. Artificial Organs 26, 307–320.

    Article  PubMed  Google Scholar 

  2. Laube, H. R., Duwe, J., Rutsch, W., and Konertz, W., (2000) Clinical experience with autologous endothelial cell-seeded polytetrafluoroethylene coronary artery bypass grafts. J. Thorac Cardiovasc. Surg. 120, 134–141.

    Article  PubMed  CAS  Google Scholar 

  3. Meinhart, J. G., Deutsch, M., Fischlein, T., Howanietz, N., Froschl, A., and Zilla P. (2001) Clinical autologous in vitro endothelialization of 153 infrainguinal ePTFE grafts. Ann. Thorac Surg. 71, S327-S331.

    Article  PubMed  CAS  Google Scholar 

  4. Heilshorn, S. C., DiZio, K. A., Welsh, E. R., and Tirrell, D. A. (2003) Endothelial cell adhesion to the fibronectin CS5 domain in artificial extracellular matrix proteins. Biomaterials 24, 4245–4252.

    Article  PubMed  CAS  Google Scholar 

  5. Tiwari, A., Salacinski, H. J., Punshon, G., Hamilton, G., and Seifalian, A. M. (2002) Development of a hybrid cardiovascular graft using a tissue engineering approach. FASEB J. 16, 791–796.

    Article  PubMed  CAS  Google Scholar 

  6. Decher, G. (1997) Fuzzy nanoassemblies: toward layered polymeric multicomposites. Science 227, 1232–1237.

    Article  Google Scholar 

  7. Ladam, G., Schaad, P., Voegel, J. C., Schaaf, P., Decher, G., and Cuisinier, F. (2000) In situ determination of the structural properties of initially deposited polyelectrolyte multilayers. Langmuir 16, 1249–1255.

    Article  CAS  Google Scholar 

  8. Shiratori, S. S., and Rubner, M. F. (2000) pH-dependent thickness behavior of sequentially adsorbed layers of weak polyelectrolytes. Macromolecules 33, 4213–4219.

    Article  CAS  Google Scholar 

  9. Picart, C., Lavalle, P. H., Hubert, P., Cuisinier, F. J. G., Decher, G., Schaaf, P., et al. (2001) Buildup mechanism for poly(L-lysine)/hyaluronic acid films onto a solid surface. Langmuir 17, 7414–7424.

    Article  CAS  Google Scholar 

  10. Richert, L., Boulmedais, F., Lavalle, P., Mutterer, J., Ferreux, E., Decher, G., et al. (2004) Improvement of stability and cell adhesion properties of polyelectrolyte multilayer films by chemical cross-linking. Biomacromolecules 5, 284–294.

    Article  PubMed  CAS  Google Scholar 

  11. Boura, C., Menu, P., Payan, E., Picart, C., Voegel, J. C., Muller, S., et al. (2003) Endothelial cells grown on thin polyelectrolyte multilayered films: an evaluation of a new versatile surface modification. Biomaterials 24, 3521–3530.

    Article  PubMed  CAS  Google Scholar 

  12. Hubbard, A. and Rothlein, R. (2000) Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radical Bio. Med. 28, 1379–1386.

    Article  CAS  Google Scholar 

  13. Tsuboi, H., Ando, J., Korenaga, R., Takada, Y., and Kamiya, A. (1995) Flow stimulates ICAM-1 expression time and shear stress dependently in cultured human endothelial cells. Biochem. Biophys. Res. Commun. 3, 988–996.

    Article  Google Scholar 

  14. Jensen, N., Lindblad, B., Ljungberg, J., Leide, S., and Bergqvist, D. (1996) Early attachment of platelets, leukocytes, and fibrinogen in endothelial cell seeded Dacron grafts. Ann. Vasc. Surg. 10, 530–536

    Article  PubMed  CAS  Google Scholar 

  15. Imbert, E., Poot, A. A., Figdor, C. G., and Feigen, J. (2001) Expression of leukocyte adhesion molecules by endothelial cells seeded on various polymer surfaces. J. Biomed. Mater. Res. 56, 376–381.

    Article  PubMed  CAS  Google Scholar 

  16. Jaffe, E. A., Nachman, R. L., Becher, C. G., and Minick, C. R. (1973) Culture of human endothelial cells derived from umbilical veins. J. Clin. Invest. 52, 2745–2756.

    Article  PubMed  CAS  Google Scholar 

  17. D'Alessio, P., Moutet, M., Coudrier, E., Darquenne, S., and Chaudiere, J. (1998) ICAM-1 and VCAM-1 expression induced by TNF-α are inhibited by a glutathione peroxidase mimic. Free Radical Bio. Med. 24, 979–987.

    Article  Google Scholar 

  18. Sun, R. J., Muller, S., Stoltz, J. F., and Wang, X. (2002) Shear stress induces caveolin-1 translocation in cultured endothelial cells. Eur. Biophys. J. 30, 605–611.

    Article  PubMed  CAS  Google Scholar 

  19. Vautier, D., Karsten, V., Egles, C., Chluba, J., Schaaf, P., Voegel, J. C., et al. (2002) Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. J. Biomat. Sci. Polym. E. 13, 713–732.

    CAS  Google Scholar 

  20. Tryoen-Toht, P., Vautier, D., Haikel, Y., Voegel, J. C., Schaaf, P., Chluba, J. et al. (2002) Viability, adhesion, and bone phenotype of osteoblast-like cells on polyelectrolyte multilayer films. J. Biomed. Mater. Res. 60, 657–667.

    Article  CAS  Google Scholar 

  21. Mutin, M., Dignat-George, F., and Sampol, J. (1997) Immunologic phenotype of cultured endothelial cells: quantitative analysis of cell surface molecules. Tissue Antigens 50, 449–458.

    Article  PubMed  CAS  Google Scholar 

  22. Mendelsohn, J. D., Barrett, C. J., Chan, V. V., Pal, A. J., Mayes, A. M., and Rubner, M. F. (2000) Fabrication of microporous thin films from polyelectrolyte multilayers. Langmuir 16, 5017–5023.

    Article  CAS  Google Scholar 

  23. Fery, A., Schöler, B., Cassagneau, T., and Caruso, F. (2001) Nanoporous thin films formed by salt-induced structural changes in multilayers of poly(acrylic acid) and poly(allylamine). Langmuir 17, 878–882.

    Article  CAS  Google Scholar 

  24. Yasuda, M., Tanaka, Y., Tamura, M., Fujii, K., Sugaya, M., So, T., et al. (2001) Stimulation of beta1 integrin down-regulates ICAM-1 expression and ICAM-1-dependent adhesion of lung cancer cells through focal adhesion kinase. Cancer Res. 61, 2022–2030.

    PubMed  CAS  Google Scholar 

  25. Nakayamada, S., Okada, Y., Saito, K., Tamura, M., and Tanaka, Y. (2003) Beta1 integrin/focal adhesion kinase-mediated signaling induces intercellular adhesion molecule 1 and receptor activator of nuclear factor kappaB ligand on osteoblasts and osteoclast maturation. J. Biol. Chem. 278, 45368–45374.

    Article  PubMed  CAS  Google Scholar 

  26. Boura, C., Muller, S., Vautier, D., Dumas, D., Schaaf, P., Voegel, J.-C., et al. (2005) Endothelial cell—interactions with polyelectrolyte multilayer films. Biomaterials 26, 4568–4575.

    Article  PubMed  CAS  Google Scholar 

  27. Carpen, O., Pallai, P., Staunton D. E., and Springer, T. A. (1992) Association of intercellular adhesion molecule-1 (ICAM-1) with actin-containing cytoskeleton and α-actinin. J. Cell Biol. 118, 1223–1234.

    Article  PubMed  CAS  Google Scholar 

  28. Schnittler, H. J., Schneider S., Raifer, H., Luo, F., Dietrich, P., Just, I., et al. (2001) Role of actin filaments in endothelial cell-cell adhesion and membrane stability under fluid shear stress. Eur. J. Physiol. 442, 675–687.

    Article  CAS  Google Scholar 

  29. Cunningham K. S. and Gotlieb A. I. (2005) The role of shear stress in the pathogenesis of atherosclerosis. Lab. Invest. 85, 9–23.

    Article  PubMed  CAS  Google Scholar 

  30. Imberti, B., Morigi, M., Zoja, C., Angioletti, S., Abbate, M., Remuzzi, A., et al. (2000) Shear stress-induced cytoskeleton rearrangement mediates NF-k-B-dependent endothelial expression of ICAM-1. Microvasc. Res. 60, 182–188.

    Article  PubMed  CAS  Google Scholar 

  31. Norgauer, J., Bartels, F., Gohring, U., Schopf, E., Gebicke-Haerter, P., Kownatzki, E., et al. (1995) Expression of intercellular adhesion molecule-1 is regulated by the actin network in epidermoid carcinoma cells. Exp. Cell Res. 219, 15–20.

    Article  PubMed  CAS  Google Scholar 

  32. Ikewaki, N., Yamada, A., and Inoko, H. (2003) Depolymerization of actin filament by cytochalasin E induces interleukin-8 production and up-regulates CD54 in the HeLa epithelial cell line. Microbiol. Immunol. 47, 775–783.

    PubMed  CAS  Google Scholar 

  33. Barreiro, O., Yanez-Mo, M., Serrador, J. M., Montoya, M. C., Vicente-Manzanares, M., Tejedor, R., et al. (2002) Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytes. J. Cell. Biol. 157, 1233–1245.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Cédric Boura.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boura, C., Muller, S., Voegel, JC. et al. Influence of polyelectrolyte multilayer films on the ICAM-1 expression of endothelial cells. Cell Biochem Biophys 44, 223–231 (2006). https://doi.org/10.1385/CBB:44:2:223

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/CBB:44:2:223

Index Entries

Navigation