Skip to main content
SpringerLink
Log in

Influence of a new axial impeller on K L a and xylanase production by Penicillium canescens 10-10c

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The effects of a new axial impeller (HTPG4) on oxygen volumetric transfer coefficient, K L a, and xylanase production by Penicillium canescens 10-10c were studied and compared for dual-impeller systems, one with one DT4 impeller below and one HTPG4 above (DT4-HTPG4) and one with two DT4 (DT4-DT4) impellers, in a 5-L bioreactor. The volumetric coefficient of oxygen transfer was measured in culture medium using a gassing-out method at different gassing rates and agitation speeds. We observed that the DT4-HTPG4 combination provided better K L a performance than the DT4-DT4 combination. The two combinations were also tested for their influence on xylanase production by a filamentous microorganism; P. canescens 10-10c. These experiments demonstrated that the DT4-HTPG4 combination impeller enhanced enzyme production up to 23% compared with the DT4-DT4 combination at an aeration rate of 1 vvm and an agitation speed of 600 rpm. The main cause for this difference is thought to be a higher shear stress generated by the DT4-DT4 combination, which damages the mycelium of P. canescens and decreases xylanase production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gogate, P. R., Beenackers, A. A. C. M., and Pandit, A. B. (2000), Biochem. Eng. J. 6, 109–144.

    Article  CAS  Google Scholar 

  2. Wang, S. J. and Zhong, J. J. (1996), Biotechnol. Bioeng. 51, 520–527.

    Article  CAS  Google Scholar 

  3. Amanullah, A., Carreon, L. S., Castro, B., Galindo, E., and Nienow, A. W. (1998), Biotechnol. Bioeng. 57, 198–210.

    Article  CAS  Google Scholar 

  4. Nienow, A. W. (1990), in Proceedings of the 5th European Congress on Biotechnology, vol. 2, Christiansen, C., Munck, L., and Valladsen, J., eds. Munksgaard International Publisher, Copenhagen, Denmark, pp. 791–796.

    Google Scholar 

  5. Lehn, M. C., Myers, K. J., and Bakker, A. (1999), Can. J. Chem. Eng. 77, 1065–1071.

    CAS  Google Scholar 

  6. McFarlanne, C. M. and Nienow, A. W. (1996), Biotechnol. Prog. 12, 9–15.

    Article  Google Scholar 

  7. Vrabel, P., Van der Lans, R. G. J. M., Luyben, K. C. A. M., Boon, L., and Nienow, A. W. (2000), Chem. Eng. Sci. 55, 5881–5896.

    Article  CAS  Google Scholar 

  8. Myers, K. J., Thomas, A. J., Bakker, A., and Reeder, M. F. (1999), Trans. IchemE 77(A), 728–730.

    Article  CAS  Google Scholar 

  9. Nienow, A. W. (1996), Trans. IchemE 74(A), 417–423.

    CAS  Google Scholar 

  10. Mishra, V. P., Dyster, K. N., Jaworski, Z., Nienow, A. W., and Mckemmie, J. (1998), Can. J. Chem. Eng. 76, 577–588.

    CAS  Google Scholar 

  11. Wase, D. A. J., McManamey, W. J., Raymahasay, S., and Vaid, A. K. (1985), Biotechnol. Bioeng. 27, 1166–1172.

    Article  CAS  Google Scholar 

  12. Panda, T. (1989), Process Biochem. 24(3), 104–108.

    CAS  Google Scholar 

  13. Palma, M. B., Milagres, A. M. F., Prata, A. M. R., and de Mancilha, I. M. (1996), Process Biochem. 31, 141–145.

    Article  CAS  Google Scholar 

  14. Gaspar, A., Strodiot, L., and Thonart, P. (1998), Appl. Biochem. Biotechnol. 70–72, 535–545.

    Google Scholar 

  15. Gaspar, A., Cosson, T., Roques, C., and Thonart, P. (1997), Appl. Biochem. Biotechnol. 67, 45–58.

    CAS  Google Scholar 

  16. Ozbek, B. and Gayik, S. (2001), Process Biochem. 36, 729–741.

    Article  CAS  Google Scholar 

  17. Schell, D. J., Farmer, J., Hamilton, J., Lyons, B., McMillan, J. D., Saez, J. C., and Tholudur, A. (2001), Appl. Biochem. Biotechnol. 91–93, 627–642.

    Article  Google Scholar 

  18. Bailey, M. J., Biely, P., and Poutanen, K. (1992), J. Biotechnol. 23, 257–270.

    Article  CAS  Google Scholar 

  19. Miller, G. L. (1959), Anal. Chem. 31, 426–428.

    Article  CAS  Google Scholar 

  20. Myers, K., Reeder, M., Bakker, A., and Rigden, M. (1996), Chem. Eng. 620, 39–42.

    CAS  Google Scholar 

  21. Arjunwadkar, S. J., Saravanan, K., Pandit, A. B., and Kulkarmi, P. R. (1998), Biochem. Eng. J. 1, 25–30.

    Article  Google Scholar 

  22. Arjunwadkar, S. J., Saravanan, K., Kulkarmi, P. R., and Pandit, A. B. (1998), Biochem. Eng. J. 1, 99–106.

    Article  CAS  Google Scholar 

  23. Heinz, P., Michael, S., and Walter, S. (1993), Biotechnol. Lett. 15, 405–410.

    Article  Google Scholar 

  24. Gomes, J., Purkarthofer, H., Hayn, M., Kappelmuller, J., Sinner, M., and Steiner, W. (1993), Appl. Microbiol. Biotechnol. 39, 700–707.

    Article  CAS  Google Scholar 

  25. Hoq, M. M., Hempel, C., and Deckwer, W. D. (1994), J. Biotechnol. 37, 49–58.

    Article  CAS  Google Scholar 

  26. Thomas, C. R. (1992), Trends Biotechnol. 10, 343–348.

    Article  CAS  Google Scholar 

  27. Leong-Poi, L. and Allen, D. G. (1992), Biotechnol. Bioeng. 40, 403–412.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre Wallon de Biologie Industrielle, Faculté Universitaire des Sciences Agronomiques, 2, Passage des Déportés, 5030, Gembloux, Belgium

    Yasser Bakri, Philippe Jacques & Philippe Thonart

  2. Pierre Guerin S.A., 179, Grand Rue, BP12, 79210, Mauze, France

    Lin Kui Shi

Authors
  1. Yasser Bakri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Philippe Jacques
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lin Kui Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Philippe Thonart
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Philippe Jacques.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bakri, Y., Jacques, P., Shi, L.K. et al. Influence of a new axial impeller on K L a and xylanase production by Penicillium canescens 10-10c. Appl Biochem Biotechnol 98, 1037–1048 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:1037

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1385/ABAB:98-100:1-9:1037

Index Entries

Search

Navigation