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MicroRNA Protocols pp 229–240Cite as

Identifying MicroRNA Regulators of Cell Death in Drosophila

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 342))

Abstract

Animal genomes contain on the order of at least hundreds of microRNAs (miRNAs). Although most remain uncharacterized, it is already clear that miRNAs regulate many bio-logical processes. A number of Drosophila miRNAs have been identified as likely cell death regulators, but functions for most have simply not been explored. Here we describe a protocol for identifying miRNAs that can act as cell death regulators. We also describe a simple protocol for testing roles for mRNAs identified as candidate miRNA targets using computational or other approaches.

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Author information

Authors and Affiliations

  1. Division of Biology, California Institute of Technology, Pasadena, CA

    Chun-Hong Chen & Bruce A. Hay

  2. Department of Neurology, Brain Research Institute, The David Geffen School of Medicine at UCLA, Los Angeles, CA

    Ming Guo

Authors
  1. Chun-Hong Chen
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  2. Ming Guo
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  3. Bruce A. Hay
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Editor information

Editors and Affiliations

  1. Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA

    Shao-Yao Ying

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© 2006 Humana Press Inc.

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Chen, CH., Guo, M., Hay, B.A. (2006). Identifying MicroRNA Regulators of Cell Death in Drosophila . In: Ying, SY. (eds) MicroRNA Protocols. Methods in Molecular Biology™, vol 342. Humana Press. https://doi.org/10.1385/1-59745-123-1:229

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  • DOI: https://doi.org/10.1385/1-59745-123-1:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-581-1

  • Online ISBN: 978-1-59745-123-9

  • eBook Packages: Springer Protocols

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