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Protein-Ligand Interaction Probed by Time-Resolved Crystallography

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Protein-Ligand Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 305))

Abstract

Time-resolved (TR) crystallography is a unique method for determining the structures of intermediates in biomolecular reactions. The technique reached its mature stage with the development of the powerful third-generation synchrotron X-ray sources, and the advances in data processing and analysis of timeresolved Laue crystallographic data. A time resolution of 100 ps has been achieved and relatively small structural changes can be detected even from only partial reaction initiation. The remaining challenge facing the application of this technique to a broad range of biological systems is to find an efficient and rapid, system-specific method for the reaction initiation in the crystal. Other frontiers for the technique involve the continued improvement in time resolution and further advances in methods for determining intermediate structures and reaction mechanisms. The time-resolved technique, combined with trapping methods and computational approaches, holds the promise for a complete structure-based description of biomolecular reactions.

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Authors and Affiliations

  1. Department of Physics, Technical University of Munich, Garching, Germany

    Marius Schmidt

  2. Department of Chemistry and School of Molecular Science, KAIST, Daejeon, South Korea

    Hyotcherl Ihee

  3. Consortium for Advanced Radiation Sources, The University of Chicago, Chicago, IL, USA

    Reinhard Pahl

  4. Consortium for Advanced Radiation Sources and Department of Biochemistry and Molecular Biology, Chicago, IL, USA

    Vukica Šrajer

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  1. Marius Schmidt
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  2. Hyotcherl Ihee
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  3. Reinhard Pahl
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  4. Vukica Šrajer
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Editors and Affiliations

  1. Department of Biophysics, University of Ulm, Ulm, Germany

    G. Ulrich Nienhaus

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Schmidt, M., Ihee, H., Pahl, R., Šrajer, V. (2005). Protein-Ligand Interaction Probed by Time-Resolved Crystallography. In: Ulrich Nienhaus, G. (eds) Protein-Ligand Interactions. Methods in Molecular Biology, vol 305. Humana, Totowa, NJ. https://doi.org/10.1385/1-59259-912-5:115

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  • DOI: https://doi.org/10.1385/1-59259-912-5:115

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-1-58829-372-5

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