Abstract
This chapter describes the use of microscope-based fluorescence resonance energy transfer to follow dynamic interaction of molecules localized at focal adhesions. We first outline the significance of studying dynamic interactions in focal adhesions of living cells, and second provide an overview of the method itself. This is followed by a protocol for fluorescence resonance energy transfer measurements in live cells.
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Ballestrem, C., Geiger, B. (2005). Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells. In: Guan, JL. (eds) Cell Migration. Methods in Molecular Biology™, vol 294. Humana Press. https://doi.org/10.1385/1-59259-860-9:321
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DOI: https://doi.org/10.1385/1-59259-860-9:321
Publisher Name: Humana Press
Print ISBN: 978-1-58829-382-4
Online ISBN: 978-1-59259-860-1
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