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Methods to Study Protein-Protein Interactions

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Epidermal Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 289))

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Abstract

Protein-protein interactions are the underpinnings of a vast number of cellular processes. In recent years, the convergence of biochemistry, cellular, and molecular biology has made available a number of powerful techniques for studying such interactions. These techniques vary in their sensitivity, efficiency, and rapidity, but judicial deployment of a combination of them has proved to be effective and reliable. Here, we highlight a version of the yeast two-hybrid assay originally pioneered by Fields and Song (1989) and subsequently enhancements by other investigators. We also briefly describe a number of new fluorescent imaging-based biophysical techniques for studying protein-protein interactions—FRET, FCS, and BiFC. Together, these constitute an impressive collection of tools for studying interactions among proteins.

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Author information

Authors and Affiliations

  1. Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine, Cincinnat, OH

    Jin-Jun Meng, Meghan Rojas, Willis Bacon, John T. Stickney & Wallace Ip

Authors
  1. Jin-Jun Meng
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  2. Meghan Rojas
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  3. Willis Bacon
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  4. John T. Stickney
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  5. Wallace Ip
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Editor information

Editors and Affiliations

  1. Ottawa Health Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2005 Humana Press Inc.

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Meng, JJ., Rojas, M., Bacon, W., Stickney, J.T., Ip, W. (2005). Methods to Study Protein-Protein Interactions. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology™, vol 289. Humana Press. https://doi.org/10.1385/1-59259-830-7:341

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  • DOI: https://doi.org/10.1385/1-59259-830-7:341

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-267-4

  • Online ISBN: 978-1-59259-830-4

  • eBook Packages: Springer Protocols

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