Abstract
Small interfering RNA (siRNA) technology has emerged as a powerful genetic tool to investigate gene function in mammalian cells. Here we use siRNA to study a mediator of DNA damage-checkpoint protein 1 (MDC1), previously known as Kiaa0170 or NFBD1, in DNA damage responses. We show that MDC1 siRNA specifically and efficiently down-regulates MDC1 expression, resulting in defective radiation-induced apoptosis in A549 cells. Transfection with siRNA-resistant MDC1 restores radiation-induced apoptosis. These findings suggest that siRNA can be a very useful tool for the exploration of gene function in mammalian checkpoint responses.
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Lou, Z., Chen, J. (2004). Use of siRNA to Study the Function of MDC1 in DNA Damage Responses. In: Schönthal, A.H. (eds) Checkpoint Controls and Cancer. Methods in Molecular Biology, vol 281. Humana Press. https://doi.org/10.1385/1-59259-811-0:179
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DOI: https://doi.org/10.1385/1-59259-811-0:179
Publisher Name: Humana Press
Print ISBN: 978-1-58829-500-2
Online ISBN: 978-1-59259-811-3
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