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Experimental Autoimmune Encephalomyelitis

  • Protocol
Autoimmunity

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 102))

Summary

Exerimental autoimmune encephalomyelitis (EAE) is an inflammatory demyleinating disease of the central nervous system that is induced in laboratory animals by the generation of an immune response against myelin epitopes. It has been used as a prototype of Th1-driven, organ-specific autoimmunity and as a model for the human disease multiple sclerosis. This chapter describes two classic protocols for EAE induction (active immunization and adoptive transfer). Subheading 3.3. describes methods for rating clinical disease in symptomatic animals. Subheading 3.4. includes instructions for the isolation of mononuclear cells from the inflamed spinal cords from mice with EAE.

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Authors and Affiliations

  1. Department of Neurology, University of Rochester School of Medicine and Dentistry, Rochester, NY

    Praveen Rao & Benjamin M. Segal

Authors
  1. Praveen Rao
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  2. Benjamin M. Segal
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Editor information

Editors and Affiliations

  1. Departments of Medicine, Microbiology, and Immunology, State University of New York, Upstate Medical University, College of Medicine, Syracuse, NY

    Andras Perl MD, PhD

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© 2004 Humana Press Inc., Totowa, NJ

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Rao, P., Segal, B.M. (2004). Experimental Autoimmune Encephalomyelitis. In: Perl, A. (eds) Autoimmunity. Methods in Molecular Medicine™, vol 102. Humana Press. https://doi.org/10.1385/1-59259-805-6:363

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  • DOI: https://doi.org/10.1385/1-59259-805-6:363

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-231-5

  • Online ISBN: 978-1-59259-805-2

  • eBook Packages: Springer Protocols

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