Abstract
Mutant cell lines provide an excellent model for studying the structure, assembly and function of proteoglycans under the controlled conditions of tissue culture. Numerous proteoglycan-deficient strains have been isolated, mostly in Chinese hamster ovary cells, and in many cases the defects have been characterized both genetically and biochemically (see Table 1). Biochemical analysis of the mutants has confirmed that various enzyme activities detected in cell-free extracts using synthetic substrates actually play a role in proteoglycan assembly in vivo. The cell lines have allowed investigators to study how altering the composition of proteoglycans affects fundamental properties of cells, such as adhesion and signaling. Moreover, animal cell mutants provide the background for predicting the phenotype of organismal mutants defective in proteoglycan assembly.
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© 2001 Humana Press Inc., Totowa, NJ
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Bai, X., Crawford, B., Esko, J.D. (2001). Selection of Glycosaminoglycan-Deficient Mutants. In: Iozzo, R.V. (eds) Proteoglycan Protocols. Methods in Molecular Biology™, vol 171. Humana Press. https://doi.org/10.1385/1-59259-209-0:309
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DOI: https://doi.org/10.1385/1-59259-209-0:309
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Print ISBN: 978-0-89603-759-5
Online ISBN: 978-1-59259-209-8
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