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Assays for Microtubule-Destabilizing Kinesins

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Book cover Kinesin Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 164))

Abstract

The kinesin superfamily is a large family of microtubule (MT)-stimulated ATPases that couple the energy of ATP hydrolysis to force production (reviewed in refs. 1 and 2). Conventional kinesin was identified as a motor protein that could translocate along MT polymers. Since the molecular cloning of kinesin (3), well over 100 additional proteins have been identified that share high sequence homology with the catalytic domain of kinesin. Many of these kinesin-related proteins (KRPs) have also been shown to be MT-based motor proteins using in vitro motility assays (reviewed in refs. 2, see also chapter 5, chapter 8, and chapter 12).

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© 2001 Humana Press Inc.

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Desai, A., Walczak, C.E. (2001). Assays for Microtubule-Destabilizing Kinesins. In: Vernos, I. (eds) Kinesin Protocols. Methods in Molecular Biology™, vol 164. Humana Press. https://doi.org/10.1385/1-59259-069-1:109

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  • DOI: https://doi.org/10.1385/1-59259-069-1:109

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-766-3

  • Online ISBN: 978-1-59259-069-8

  • eBook Packages: Springer Protocols

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