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Methods for Gene Transfer to Synovium

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Gene Therapy Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 7))

Abstract

Development of methods for gene transfer to synoviocytes was borne from the idea that gene therapy could be used to more effectively treat rheumatoid arthritis (EU) and other joint disorders (1). Current pharmaceutical modalities in use against RA have limited effectiveness because of problems related to inefficient targeting of drugs to the joint, as well as inefficacies of the drugs themselves. Drug delivery to the joint by traditional oral, iv, and intramuscular routes, depends on passive diffusion of the drug from the synovial vasculature into the joint space (2). Thus, high systemic concentrations of the drug are necessary to achieve therapeutic intra-articular drug levels; in chronic RA, perfusion of the synovium may be compromised (3), driving required systemic drug levels even higher. This is of major concern, as the pharmaceuticals used to treat this disease are associated with serious side effects. Further compounding these problems is the chronic nature of RA, which requires lifelong treatment with high dosages of these drugs.

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Author information

Authors and Affiliations

  1. Orthopedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Richard Kang

  2. Department of Molecular Genetics and Biochemistry, University of Pittsburgh, PA, USA

    Paul D. Robbins

  3. Department of Molecular Genetics and Biochemistry, University of Pittsburgh Medical Center, Pittsburgh, PA, USA

    Christopher H. Evans

Authors
  1. Richard Kang
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  2. Paul D. Robbins
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  3. Christopher H. Evans
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Editor information

Editors and Affiliations

  1. University of Pittsburgh, PA, USA

    Paul D. Robbins

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© 1997 Humana Press Inc., Totowa, NJ

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Kang, R., Robbins, P.D., Evans, C.H. (1997). Methods for Gene Transfer to Synovium. In: Robbins, P.D. (eds) Gene Therapy Protocols. Methods in Molecular Medicine, vol 7. Humana, Totowa, NJ. https://doi.org/10.1385/0-89603-484-4:357

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  • DOI: https://doi.org/10.1385/0-89603-484-4:357

  • Publisher Name: Humana, Totowa, NJ

  • Print ISBN: 978-0-89603-484-6

  • Online ISBN: 978-1-59259-591-4

  • eBook Packages: Springer Protocols

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