Abstract
In whole mount in situ hybridization, the distribution of RNA transcripts is visualized in the intact embryo. This allows for the rapid realization of transcript distribution throughout the embryo without the need for reconstruction from sections. In addition, embryos probed for different transcripts (or for the same transcript under different experimental conditions) can be readily compared. The procedure is limited to stages where the embryos are large enough to be conveniently processed, but not so large as to prevent penetration of the reagents (embryonic d 6–10 in the mouse). The following procedure for mouse embryos (1) is derived from whole mount in situ hybridization procedures for the embryos of other species (2,3). This procedure also can be applied to isolated embryonic organs and tissues (4).
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References
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© 1997 Humana Press Inc., Totowa, NJ
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Conlon, R.A. (1997). Whole Mount In Situ Hybridization to Embryos and Embryonic Tissues. In: Tuan, R.S. (eds) Recombinant Protein Protocols. Methods in Molecular Biology™, vol 63. Humana Press. https://doi.org/10.1385/0-89603-481-X:257
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DOI: https://doi.org/10.1385/0-89603-481-X:257
Publisher Name: Humana Press
Print ISBN: 978-0-89603-481-5
Online ISBN: 978-1-59259-549-5
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