Abstract
The use of an electrical field to permeabilize cells reversibly (electroporation) has become a valuable technique for transference of DNA into both eukaryotic and prokaryotic cells. Many species of bacteria have been successfully electroporated (1) and many strains of E. coli are routinely electrotransformed to efficiencies of 109 and 1010 transformants/µg DNA. Frequencies of transformation can be as high as 80% of the surviving cells and DNA capacities of nearly 10 µg of transforming DNA/mL are possible (2).
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Drury, L. (1996). Transformation of Bacteria by Electroporation. In: Harwood, A.J. (eds) Basic DNA and RNA Protocols. Methods in Molecular Biology™, vol 58. Humana Press. https://doi.org/10.1385/0-89603-402-X:249
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DOI: https://doi.org/10.1385/0-89603-402-X:249
Publisher Name: Humana Press
Print ISBN: 978-0-89603-402-0
Online ISBN: 978-1-59259-251-7
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