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The Crystallization and Structure Analysis of Oligonucleotide Sequences

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Crystallographic Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 56))

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Abstract

Until 1976, the study of nucleic acid structure was exclusively the domain of fiber diffractionists. Between the original Watson-Crick structure in 1953 and this date, there was considerable activity in refining the original B-form model of DNA and extending the approach to other polymorphs and a number of synthetic, repetitious polynucleotides, all of which were based on data from fiber-diffraction samples. These studies reached their zenith with the development and use of a “linked-atom,” least-squares refinement procedure for the optimization of mono-or dinucleotide repeat units against the relatively sparse para-crystalline diffraction data from ordered fibers. It is a tribute to the sophistication of these analyses, in spite of the inherent limitations of fiber data, that the refined “canonical” A- and B-DNA double helices are still major reference pomts for many studies (1).

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Authors and Affiliations

  1. CRC Biomolecular Structure Unit, The Institute of Cancer Research, Sutton, UK

    Stephen Neidle

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  1. Stephen Neidle
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Editors and Affiliations

  1. National Institute for Biological Standards and Control, Potters Bar, UK

    Christopher Jones  & Barbara Mulloy  & 

  2. Department of Biophysics, King’s College, London, UK

    Mark R. Sanderson

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Neidle, S. (1996). The Crystallization and Structure Analysis of Oligonucleotide Sequences. In: Jones, C., Mulloy, B., Sanderson, M.R. (eds) Crystallographic Methods and Protocols. Methods in Molecular Biology™, vol 56. Humana Press. https://doi.org/10.1385/0-89603-259-0:267

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  • DOI: https://doi.org/10.1385/0-89603-259-0:267

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-259-0

  • Online ISBN: 978-1-59259-543-3

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