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Plant Cell and Tissue Culture pp 39–48Cite as

Cryopreservation of Plant Cells

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 6))

Abstract

Cryopreservation, that is, the viable storage of cells at the temperature of liquid nitrogen (-196°C), has wide relevance in many areas of pure and applied biology. Examples of its very successful use can be found in the storage of microbes and of semen (1). More recently, attention has been given to the development of cryopreservation methods for embryos, including those of humans, tissues, and organs for transplantation and blood components. In the context of plant research, realization of the potential applications of cryopreservation has been somewhat latent. However, several clear areas for application can be identified that involve the need to store exact genotypes in a stable state.

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Author information

Authors and Affiliations

  1. University of Nottingham, Nottingham, UK

    Lgndsey A. Withers

Authors
  1. Lgndsey A. Withers
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Editor information

Editors and Affiliations

  1. Albert Einstein College of Medicine, Bronx, New York

    Jerey W. Pollard

  2. The Hatfield Polytechnic, Hatfield, Hertfordshire, UK

    John M. Walker

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© 1990 Humana Press Inc.

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Withers, L.A. (1990). Cryopreservation of Plant Cells. In: Pollard, J.W., Walker, J.M. (eds) Plant Cell and Tissue Culture. Methods in Molecular Biology™, vol 6. Humana Press. https://doi.org/10.1385/0-89603-161-6:39

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  • DOI: https://doi.org/10.1385/0-89603-161-6:39

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-161-6

  • Online ISBN: 978-1-59259-493-1

  • eBook Packages: Springer Protocols

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