Orientation-Controlled Electrocatalytic Efficiency of an Adsorbed Oxygen-Tolerant Hydrogenase
Fig 2
Voltammetric traces of Re MBH immobilized on SAM-coated Au electrodes in the presence of H2-saturated buffer
: (A) measured before (black) and after (blue) addition of methylene blue; (B) measured before (red) and after (blue) addition of methylene blue, but subsequent to a 5 min pre-activation step at −340 mV in the presence of H2. All voltammetric measurements were carried out at room temperature with a scan rate of 5 mV s−1. (C) SEIRA spectra of the amide region recorded before (solid black), after (dashed black), and during (red) the pre-activation at −340mV under H2 atmosphere. The inset shows the corresponding stretching vibration of the CO ligand at the active site prior (solid line) and after (dashed line) pre-activation in the oxidized state. Black spectra (vide supra) were recorded at OCP (+260 mV). Potentials are given with respect to the standard hydrogen electrode (SHE).