Optical Imaging of Paramagnetic Bead-DNA Aggregation Inhibition Allows for Low Copy Number Detection of Infectious Pathogens
Fig 6
Detecting the presence of DNA specific to the human thyroid peroxidase (TPOX) gene and Rift Valley fever virus (RVFV) RNA.
A) PiBA for detection of human-specific DNA from whole blood. Primers were designed to target the TPOX gene. B) RVFV detection from infected (viral RNA in a background of host RNA) samples. #1–4 represent blinded viral samples. C) Results of blinded analysis of samples. D) Results suggesting that “% Inhibition of Aggregation” is concentration dependent and varies with time of amplification.