Inhibition of Mitochondrial Complex III Blocks Neuronal Differentiation and Maintains Embryonic Stem Cell Pluripotency
Figure 1
Antimycin A disturbs mESC neuronal differentiation.
mESCs differentiated for 14 days into dopaminergic neurons using a PA6-based system were treated with AA since day 2. Representative phase contrast microscopy images were obtained at different time-points of the process (days 7, 10 and 14). AA-treated colonies were smaller and with a more compact morphology resembling undifferentiated mES cells colonies. AA-treatment also led to the absence of neuronal processes, which are easily depicted in control (CTR) colonies at later stages.