A Selective HDAC 1/2 Inhibitor Modulates Chromatin and Gene Expression in Brain and Alters Mouse Behavior in Two Mood-Related Tests
Figure 5
Chromatin immunoprecipitation (ChIP) of nucleus accumbens from Cpd-60 treated mice.
(a) Schematic of experimental design with 10-day administration of Cpd-60 (45 mg/kg, i.p.). (b) Chromatin was immunoprecipitated with an anti-histone H4K12ac-antibody followed by qPCR targeting regions 1.0 or 0.2 kB upstream or 0.5 kB downstream from the transcription start site (TSS). Transcripts upregulated by Cpd-60 treatment had increased histone acetylation at promoter regions upstream, but not downstream, of the TSS in Cpd-60 treated tissue compared to vehicle. *p<0.05, **p<0.01, t-test of Cpd-60 versus vehicle.