The Reno-Vascular A2B Adenosine Receptor Protects the Kidney from Ischemia
Figure 8
A2BAR Agonist (BAY 60–6583). A2BAR−/− Mice or Age-, Weight-, and Gender-Matched Littermate Controls Underwent Right Nephrectomy followed by 45 min of Left Renal Artery Ischemia and 24 h of Reperfusion
They underwent treatment with BAY 60–6583 (60 μg/kg intravenous) or vehicle control given before the surgery, immediately before ischemia, 5 min or 2h after ischemia.
(A) GFR (as measured by inulin clearance and (B) Jablonski index for histological quantification of ischemic injury were obtained after 24 h of reperfusion.
(C, D) Representative HE staining (400× and 800×) of renal histology following 45 min of ischemia with (+BAY) or without BAY 60–6583 (−BAY) given prior to ischemia are displayed.
Statistics: (A) One-way ANOVA with Tukey's post-hoc test was performed (*, p < 0.01 versus the WT mice with BAY treatment, **, p < 0.001 versus WT mice with BAY treatment). The F-test results are (A) F (11,69) = 54.84, p < 0.001.
(B) Kruskal-Wallis nonparametric analysis (chi-square = 61.08, df = 11, p < 0.001) with follow-up pairwise comparisons by Wilcoxon-Mann-Whitney test was performed with *, p < 0.01 versus WT mice with BAY treatment.