Abstract
Monodisperse 3.2-μm porous poly(styrene-co-divinylbenzene) particles have been prepared by a new multistage polymerization procedure, so-called ‘modified seeded polymerization’. In the synthesis of the particles sufficient porosity was generated by use of a diluent mixture with relatively low viscosity. The use of relatively high diluent/seed latex ratio resulted in the formation of particles with an average pore size of 13 nm. The particles were used as column-packing material in HPLC and their performance was compared with that of larger particles (dn = 7.8 μm) obtained by the same method. The SEC plot for the 3.2 μm particles showed that the proposed material was particularly suitable for separation of analytes in the molecular weight range 100–2000. In reversed-phase mode alkylbenzenes were separated with resolution >2 by use of a relatively small column (50 mm × 4.6 mm i.d.) packed with 3.2-μm particles. Theoretical plate numbers (TPN) in excess of 35 000 plates m−1 could be achieved with benzene as analyte. Whereas the ratio of TPN determined with pentylbenzene to that determined with benzene was less than 0.7 for the 7.8-μm particles, the same ratio was 0.95 for the 3.2-μm particles. With the 3.2-μm packing material proposed no significant decrease in resolving power was observed when the mobile-phase flow rate was increased sevenfold.
Similar content being viewed by others
References
Ugelstad J (1978) Macromol Chem 179:815–817
Ugelstad J, Kaggerud KH, Hansen FK, Berge A (1979) Macromol Chem 180:737–744
Ellingsen T, Aune O, Ugelstad J, Hagen S (1990) J Chromatogr 535:147–161
Cheng CM, Micale FJ, Vanderhoff JW, El-Aasser MS (1992) J Polym Sci, Part A: Polym Chem 30:235–244
Cheng CM, Vanderhoff JW, El-Aasser MS (1992) J Polym Sci, Part A: Polym Chem 30:245–256
Galia M, Svec F, Frechet JMJ (1994) J Polym Sci, Part A: Polym Chem 32:2169–2175
Smigol V, Svec F (1992) J Appl Polym Sci 46:1439–1448
Smigol V, Svec F, Frechet JMJ (1994) Anal Chem 66:4308–4315
Tuncel A, Tuncel M, Salih B (1999) J Appl Polym Sci 71:2271–2290
Tuncel A (1999) J Appl Polym Sci 71: 2291–2302
Li WH, Stöver HDH (2000) Macromolecules 33:4354–4360
Hosoya K, Teramachi M, Tanaka N, Kobayashi A, Kanda T, Ohtsu Y (2001) Anal Chem 73:5852–5857
Zhang S, Huang X, Yao N, Horvath C (2002) J Chromatogr A 948:193–201
Zhang S, Zhang J, Horvath C (2002) J Chromatogr A 965:83–92
Xu M, Peterson DS, Rohr T, Svec F, Frechet JMJ (2003) Anal Chem 75:1011–1021
Camli ST, Senel S, Tuncel A (1999) J Biomater Sci, Polym Ed 10:875–889
Camli T, Tuncel M, Senel S, Tuncel A (2002) J Appl Polym Sci 84:414–429
Tuncel A, Tuncel M, Ergun B, Alagöz C, Bahar T (2002) Colloid Surface A 197:79–94
Tuncel A, Tuncel M, Cicek H, Fidanboy O (2001) Polym Int 51:75–84
Senel S, Çamli ST, Tuncel A (2002) J Chromatogr B 769:283–295
Tuncel A, Unsal E, Senel S (2003) Polymer beads in biomedical chromatography: synthesis and characterization. In: Elaissari A (Ed) Colloidal polymers: synthesis and characterization, Marcel Dekker, New York, pp 319–349
Unsal E, Camli ST, Senel S, Tuncel A (2004) J Appl Polym Sci 92:607–618
Camli ST, Unsal E, Senel S, Tuncel A (2004) J Appl Polym Sci 92:3685–3696
Unsal E, Elmas B, Camli S T, Tuncel M, Şenel S, Tuncel A (2004) J Appl Polym Sci, In press
Tuncel A, Kahraman R, Piskin E (1993) J Appl Polym Sci. 50:303–319
Tuncel A, Kahraman R, Piskin E (1994) J Appl Polym Sci 51:1485–1498
Ferreira A, Bigan M, Blondeau D (2003) React Funct Polym 56:123–136
Wang QC, Svec F, Frechet JMJ (1994) J Polym Sci, Part A: Polym Chem 32:2577–2588
Ullmann’s Encyclopedia of Industrial Chemistry (1985) John Wiley and Sons, New York
Halasz I, Martin K (1978) Angew Chem Int Ed 17:901–906
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Unsal, E., Çamlı, S., Irmak, T. et al. Monodisperse Poly (Styrene-co-Divinylbenzene) Particles (3.2 μm) with Relatively Small Pore Size as HPLC Packing Material. Chromatographia 60, 553–560 (2004). https://doi.org/10.1365/s10337-004-0416-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-004-0416-4