Abstract
Nonaqueous capillary electrophoresis coupled to UV–vis detection is employed for the fingerprint analysis of Zanthoxylum nitidum. A reliable separation of the analytes in Z. nitidum was performed by using methanol-water solution (4:1, v/v) containing 100 mM sodium acetate (pH* 5.0) as electrolyte solution. Under the conditions studied, the fingerprint of Z. nitidum was obtained within 50 min. The samples from 11 sources showed fair similarity. The fingerprint was used to identify and evaluate the consistency of the herb. The Z. nitidum was well distinguished from Z. nitidum var. fastuosum, a variety in traditional Chinese medicine by comparing the fingerprints of each herb at the same condition.
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The financial support from Science Foundation of the Key Laboratory of Medicinal Chemical Resources and Molecular Engineering of China Ministry of Education and the Innovation Foundation of Guangxi Normal University is gratefully acknowledged.
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Hu, K., Zhao, S., Ye, F. et al. Fingerprint Analysis of Zanthoxylum nitidum by Nonaqueous CE. Chroma 68, 475–479 (2008). https://doi.org/10.1365/s10337-008-0702-7
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DOI: https://doi.org/10.1365/s10337-008-0702-7