Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Simultaneous photoacoustic microscopy of microvascular anatomy, oxygen saturation, and blood flow

Not Accessible

Your library or personal account may give you access

Abstract

Capitalizing on the optical absorption of hemoglobin, photoacoustic microscopy (PAM) is uniquely capable of anatomical and functional characterization of the intact microcirculation in vivo. However, PAM of the metabolic rate of oxygen (MRO2) at the microscopic level remains an unmet challenge, mainly due to the inability to simultaneously quantify microvascular diameter, oxygen saturation of hemoglobin (sO2), and blood flow at the same spatial scale. To fill this technical gap, we have developed a multi-parametric PAM platform. By analyzing both the sO2-encoded spectral dependence and the flow-induced temporal decorrelation of photoacoustic signals generated by the raster-scanned mouse ear vasculature, we demonstrated—for the first time—simultaneous wide-field PAM of all three parameters down to the capillary level in vivo.

© 2015 Optical Society of America

Full Article  |  PDF Article
More Like This
Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

Tan Liu, Qing Wei, Jing Wang, Shuliang Jiao, and Hao F. Zhang
Biomed. Opt. Express 2(5) 1359-1365 (2011)

All-optical photoacoustic Doppler transverse blood flow imaging

Wei Qiao, Zhongjiang Chen, Wangting Zhou, and Da Xing
Opt. Lett. 43(11) 2442-2445 (2018)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved