Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Saturated Förster resonance energy transfer microscopy with a stimulated emission depletion beam: a pathway toward single-molecule resolution in far-field bioimaging

Not Accessible

Your library or personal account may give you access

Abstract

We theoretically demonstrate that the spatial resolution of stimulated emission depletion (STED) microscopy can be substantially enhanced without increasing the intensity of the STED beam. In our scheme, tiny nanobeads codoped with donor and acceptor molecules are used as fluorescent probes, in which Förster resonance energy transfer (FRET) can occur with an 100% efficiency between the donors and acceptors. Enhancement of the depletion of acceptors in the nanobeads with the doughnut-shaped depletion beam can lead to an increase of FRET efficiency in the outer area of the excitation spot, which itself is used for deexciting donor molecules and, consequently, enhancing the optical resolution.

© 2010 Optical Society of America

Full Article  |  PDF Article
More Like This
Far-field superresolution imaging with dual-dye-doped nanoparticles

Jianfang Chen and Ya Cheng
Opt. Lett. 34(12) 1831-1833 (2009)

Improved resolution in fluorescence microscopy with the FRET pairs by time gating

Shangguo Hou, Jianfang Chen, Suhui Deng, Fei Wang, Qing Huang, Ya Cheng, and Chunhai Fan
Opt. Express 23(10) 13121-13129 (2015)

Far-field lithography through saturated resonance energy transfer

Nikolaos Liaros
Opt. Lett. 47(13) 3327-3330 (2022)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (3)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (2)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved