Abstract
Using the method of gene transfection with liposome, we obtained the mouse erythroleukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation. Our biorheological study indicated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger osmotic fragility, lower cell surface charge density, increased elastic modulus K 1 which is inversely proportional to cells’ maximal deformation ability, obviously diminished surface viscosity μ, with elastic modulus K 2having no distinct changes. The above results provided some bases for recognizing the function of TFAR19 completely from the viewpoint of biorheology.
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Gu, L., Yao, W., Yan, Z. et al. Effects of TFAR19 gene on the growth and biorheological properties of mouse erythroleukemia cell line MEL. Sci. China Ser. C.-Life Sci. 46, 293–301 (2003). https://doi.org/10.1360/03yc9031
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DOI: https://doi.org/10.1360/03yc9031