Analysis of Algae Growing on the Surface of the North City Wall of Liangzhu SiteChinese Full Text

WU Fasi;WANG Wanfu;HE Dongpeng;XU Ruihong;SU Bomin;Conservation Institute,Dunhuang Academy;National Research Center for Conservation of Ancient Wall Paintings and Earthen Sites;Key Scientific Research Base for Conservation of Ancient Murals of SACH;Key Laboratory for Conservation of Ancient Wall Paintings and Earthen Sites in Gansu Province;School of Life Sciences,Lanzhou University;

Abstract: This paper seeks to better clarify the major algal groups that have caused biofouling to earthen sites preserved in humid environments and to provide evidence for studying biodeterioration while establishing appropriate countermeasures for later periods.Modern molecular biology research methods were applied for:the extraction of total DNA of the samples,amplification of the targeted fragments,construction of the clone library,determination of sequences,and analysis of phylogeny.Lab results show that three phylums and five genuses of algae might cause biofouling to the earthen sites.Among them,the Nostoc group which belongs to the phylum Cyanophyta is most dominant,the groups of Nitzschia and Phaeodactylum that belong to the phylum Bacillariophyta are less dominant,and the Microcoleus of the phylum Cyanophyta and other unidentified genus of the phylum Chlorophyta are the least dominant.In addition,most sequences retrieved from some samples are similar to Leptobryum,which belongs to the Bryopsida class of the Bryophyta phylum.The differences in community components between photosynthetic algae and mosses are greatly influenced by the spatial positions of sampling sites and by the water contents of the soil.To reduce the lighting intensity and water penetration in the environment of the earthen sites might be the most efficient way to control the photosynthetic organism erosion.

Keywords：

earthen sites; algae; mosses; clone library; community composition; humid environment;

DOI：
10.13584/j.cnki.issn1000-4106.2014.04.017
Series：
Subject：
Classification Code：
K878.3

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