Journal of Hunan Agricultural University(Natural Sciences). 2014, (05) Core

Cutting rooting characters of Macropanax rosthornii and its physiological and biochemical analysisChinese Full Text

LIANG Wen-bin;NIE Dong-ling;WU Si-zheng;BAI Weng-fu;SHEN Su-zhen;College of Life Science and Technology, Central South University of Forestry and Technology;Hunan Forest botanical Garden;

Abstract: Contrasted by water dipped samples, 3-year hard-wood cuttings of Macropanax rosthornii, dipped with IBA at a concentration of 500 mg/L, were inserted into a substrate of yellow subsoil according to completely randomized block design. Rooting characters and the changes of soluble sugar content, soluble protein content and activity of polyphenol oxidase（PPO）, peroxidase（POD）, indoleacetic acid oxidase（IAAO） were studied during cutting rooting. The anatomic mechanism of rooting was also studied by using 1-year soft-wood cuttings of Macropanax rosthornii. The results showed that the new rooting originated from cortex with a 97% rooting rate, and there was no callus formation at the base. The soluble sugar content was decreased either in root-induced stage or in early production stage, and the maximal decrement was 64.75% compared with that of pre-cutting, while, it increased in root formation stage. The soluble protein content was decreased by 69.18% in root-induced stage and increased both in root formation stage and in root elongation stage, with a maximal increment of 55.51% compared with those of pre-cutting. The activity of PPO was increased to the maximum with a 515.85% increment in root-induced stage and decreased to the lowest in root formation stage, and then increased to the maximum with a 590.24% increment in the root elongation stage compared with those of pre-cutting. The activity of POD reached its peak either in root-induced stage or in root elongation stage with a maximal increment of 59.20% compared with that of pre-cutting. The activity of IAAO was increased gradually in root-induced stage, it reached to a peak with an increment of 14.84% in root formation stage compared with that of pre-cutting, and then decreased to the pre-cutting level in root elongation stage. The root number, sugar content and protein content of cuttings in the treatment of IBA were higher than those of in the control, while, the activity of PPO, POD and IAAO were hardly affected by the treatment of exogenous IBA.

Keywords：

Macropanax rosthornii; cutting; rooting character; nutrients content; oxidase activity;

DOI：
10.13331/j.cnki.jhau.2014.05.012
Series：
Subject：
Classification Code：
S687

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Cutting rooting characters of Macropanax rosthornii and its physiological and biochemical analysisChinese Full Text

LIANG Wen-bin;NIE Dong-ling;WU Si-zheng;BAI Weng-fu;SHEN Su-zhen;College of Life Science and Technology, Central South University of Forestry and Technology;Hunan Forest botanical Garden;

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