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Progress in Modern Biomedicine. 2014, (24)

Changes in the Expression of Calcitonin Gene-Related Peptide in Rats after Formalin InjectionChinese Full Text

LI Ji-hong;HE Qian;SHI Yong-mei;LIAO Jun;LI Liang;LIU Chao-qun;CHEN An;YU Qing-ping;Department of Human Anatomy, Hunan university of Chinese Medine;

Abstract: Objective: To observe the temporospatial expression changing laws of calcitonin gene-related peptide（CGRP） in the spinal cord and dorsal root ganglion（DRG） after subcutaneouly formalin injection in rats hind planta. Methods: 54 healthy adult SD rats were distributed into physiological saline group and experimental groups, while experimental groups were subdivided randomly into 15 min, 30 min, 1 h, 3 h, 6 h, 12 h, 24 h and 72 h groups（n=6） which were treated with 5% formalin 100 μL injected into rat hind planta.The expression and distribution of CGRP in the lumber spinal cord and L4~6 DRG were detected by immunohistochemical method and image analysis technique. Results: CGRP was expressed in normal anterior and posterior horns of spinal cord and DRG. The expression of CGRP in DRG neurons was up-regulated in 3h group after subcutaneouly formalin injection. However, changes of CGRP in DRG peaked at 12 h~24 h groups, then decreased to normal level at 72 h group. The expression of CGRP increased in the lamina Ⅱ of spinal cord at 6 h group, and peaked at 12 h ~24 h groups, then decreased to normal level at 72 h group. There were no changes in the CGRP-positive spinal ventral motoneurons of all groups. Conclusions: The changes of CGRP presented a temporospatial pattern in the spinal cord and DRG after formalin injection, which may be one of mechanisms that participated in the nociceptive signal transmission.
Keywords：

Calcitonin gene-related peptide; Formalin; Dorsal root ganglion; Spinal cord;

  • DOI：

    10.13241/j.cnki.pmb.2014.24.011

  • Series：

  • Subject：

  • Classification Code：

    R965

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