BMRB Entry 34207

Title:
Backbone structure of free bradykinin (BK) in DDM/CHS detergent micelle determined by MAS SSNMR
Deposition date:
2017-11-29
Original release date:
2018-01-05
Authors:
Mao, J.; Lopez, J.; Shukla, A.; Kuenze, G.; Meiler, J.; Schwalbe, H.; Michel, H.; Glaubitz, C.
Citation:

Citation: Joedicke, Lisa; Mao, Jiafei; Kuenze, Georg; Reinhart, Christoph; Kalavacherla, Tejaswi; Jonker, Hendrik; Richter, Christian; Schwalbe, Harald; Meiler, Jens; Preu, Julia; Michel, Hartmut; Glaubitz, Clemens. "The molecular basis of subtype selectivity of human kinin G-protein-coupled receptors."  Nat. Chem. Biol. 14, 284-290 (2018).
PubMed: 29334381

Assembly members:

Assembly members:
entity_1, polymer, 9 residues, 1062.224 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: RPPGFSPFR

Data sets:
Data typeCount
13C chemical shifts22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 9 residues - 1062.224 Da.

1   ARGPROPROGLYPHESERPROPHEARG

Samples:

sample_1: Bradykinin (BK), [U-13C; 15N]-F8R9, 0.36 ug/uL; HEPES 50 mM; NaCl 100 mM; EDTA 2 mM; DDM 4 % w/v

sample_2: Bradykinin (BK), [U-13C; 15N]-P2P3G4F5S6P7F8, 0.36 ug/uL; HEPES 50 mM; NaCl 100 mM; EDTA 2 mM; DDM 4 % w/v

sample_conditions_1: ionic strength: 125 mM; pH: 7.4; pressure: 1 atm; temperature: 200 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DQ-SQsample_1isotropicsample_conditions_1
2D 13C-13C DQ-SQsample_2isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

TOPSPIN, Bruker Biospin - chemical shift assignment, collection, processing

TALOS+, Shen, Delaglio, Cornilescu, Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz