Bifidobacteria and Microflora
Online ISSN : 1884-5126
Print ISSN : 0286-9306
ISSN-L : 0286-9306
Isolation and Identification of Bifidobacterium spp.in Commercial Yogurts Sold in Europe
Hirokazu IWANAHiroko MASUDATomohiko FUJISAWAHideki SUZUKITomotari MITSUOKA
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1993 Volume 12 Issue 1 Pages 39-45

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Abstract

A new selective medium, galactose (GL) agar, was developed for the enumeration of Bifidobacterium spp. in yogurts. This medium contains lithium chloride (0.4 g/1) and galactose (10 g/l) as selective inhibitory agents. The viable counts of bifidobacteria in the commercial yogurts sold in Europe were determined by using GL agar. From five of eight commercial yogurts tested, bifidobacteria were detected in 104-107 per ml, while from the remaining three they were not detected. Five Bifidobacterium strains isolated were identified on carbohydrate fermentation patterns and DNA-DNA homology. Only one strain was classified B. animalis. and the remaining four strains as B. animalis.

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