Global metabolomic analysis of blood from mice infected with Brucella abortus

Son Hai VU; Bomin KIM; Alisha Wehdnesday Bernardo REYES; Tran Xuan Ngoc HUY; John Hwa LEE; Suk KIM; Hyun-Jin KIM

doi:10.1292/jvms.20-0630

Public Health

Global metabolomic analysis of blood from mice infected with Brucella abortus

Son Hai VU, Bomin KIM, Alisha Wehdnesday Bernardo REYES, Tran Xuan Ngoc HUY, John Hwa LEE, Suk KIM, Hyun-Jin KIM

Author information

Son Hai VU
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
Institute of Applied Sciences, Ho Chi Minh City University of Technology–HUTECH, 475A Dien Bien Phu St., Ward 25, Binh Thanh District, Ho Chi Minh City, Vietnam
Bomin KIM
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
Alisha Wehdnesday Bernardo REYES
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
Tran Xuan Ngoc HUY
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
John Hwa LEE
College of Veterinary Medicine, Chonbuk National University, Iksan, 54596, Republic of Korea
Suk KIM
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea
Hyun-Jin KIM
Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, 52828, Republic of Korea

Keywords: Brucella abortus infection, lysophosphatidylcholine, metabolomics profiling, serum, ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry

JOURNAL OPEN ACCESS

2021 Volume 83 Issue 3 Pages 482-486

DOI https://doi.org/10.1292/jvms.20-0630

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Abstract

To better understanding Brucella abortus infection, serum metabolites of B. abortus-infected and -uninfected mice were analyzed and twenty-one metabolites were tentatively identified at 3 and 14 days post-infection (d.p.i.). Level of most lysophosphatidylcholines (LPCs) was found to increase in infected mice at 3 d.p.i., while it was decreased at 14 d.p.i. as compared to uninfected mice. In contrast, acylcarnitines were initially reduced at 3 d.p.i then elevated after two-weeks of infection, while hydroxysanthine was increased at 14 d.p.i. in infected mice. Our findings suggest that the significant changes in LPCs and other identified metabolites may serve as potential biomarkers in acute phase of B. abortus infection.

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