Index

e-ISSN 1828-1427

  

Rivista trimestrale di Sanità Pubblica Veterinaria edita dall'Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise ‘G. Caporale’

A quarterly journal devoted to veterinary public health, veterinary science and medicine published by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ in Teramo, Italy

Editorial policy
Copyright and disclaimers
Guidelines for Authors
Editors
Honorary Members
Editorial Board
Scientific Advisory Board
Associate Editors
Issues online
Tariffs
Contact
 

ISSUES ONLINE

2014 - Volume 50 (4), October-December
   
 
Abdelfattah M. Selim, Mahmoud M. Elhaig & Wolfgang Gaede  
Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus 269-275
doi: 10.12834/VetIt.222.702.3
 
Summary
Abortion among dairy cattle is one of the major causes of economic losses in the livestock industry. This study describes a 1-step multiplex real-time polymerase chain reaction (PCR) to detect Brucella spp., Leptospira spp. and Campylobacter foetus, these are significant bacteria commonly implicated in bovine abortion. ß-actin was added to the same PCR reaction as an internal control to detect any extraction failure or PCR inhibition. The detection limit of multiplex real-time PCR using purified DNA from cultured organisms was set to 5 fg for Leptospira spp. and C. foetus and to 50 fg for Brucella spp. The multiplex real-time PCR did not produce any non-specific amplification when tested with different strains of the 3 pathogens. This multiplex real-time PCR provides a valuable tool for diagnosis, simultaneous and rapid detection for the 3 pathogens causing abortion in bovine.

Keywords
Abortion, Cattle, Brucella spp., Leptospira spp., Campylobacter foetus, Multiplex real-time PCR.


Full article

      
 
    © Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 2014