Identification of resistance to Fusarium head blight and molecular cytogenetics of interspecific derivatives between wheat and Psathyrostachys huashanica

Chenchen Hou; Jing Han; Liangliang Zhang; Qiang Geng; Li Zhao; Shuhui Liu; Qunhui Yang; Xinhong Chen; Jun Wu

doi:10.1270/jsbbs.21089

Research Papers

Identification of resistance to Fusarium head blight and molecular cytogenetics of interspecific derivatives between wheat and Psathyrostachys huashanica

Chenchen Hou, Jing Han, Liangliang Zhang, Qiang Geng, Li Zhao, Shuhui Liu, Qunhui Yang, Xinhong Chen, Jun Wu

Author information

Chenchen Hou
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Jing Han
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Liangliang Zhang
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Qiang Geng
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Li Zhao
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Shuhui Liu
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Qunhui Yang
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Xinhong Chen
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University
Jun Wu
Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University

Keywords: Fusarium head blight, Psathyrostachys huashanica, ditelosomic addition line, substitution line, molecular cytogenetics, marker analysis, 660K genotyping array analysis

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Supplementary material

2022 Volume 72 Issue 3 Pages 213-221

DOI https://doi.org/10.1270/jsbbs.21089

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Abstract

Psathyrostachys huashanica is a relative of wheat (Triticum aestivum L.) with many disease resistance genes that can be used to improve wheat disease resistance. In order to enrich the germplasm resources available in wheat genetics and breeding, we assessed Fusarium head blight (FHB) resistance in 45 interspecific derivatives between wheat and Psathyrostachys huashanica during two years from 2017–2018. Two interspecific derivatives comprising, H-34-8-2-6-1 and H-24-3-1-5-19-1 were identified as FHB resistant lines. These two lines were examined based on their morphology and cytogenetics, as well as by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), molecular markers, and 660K genotyping array to determine their genetic construction. The results confirmed H-34-8-2-6-1 as a wheat–P. huashanica 1Ns long arm ditelosomic addition line and H-24-3-1-5-19-1 as a wheat–P. huashanica 2Ns substitution line. Assessments of the agronomic traits showed that H-34-8-2-6 had significantly higher kernel number per spike and self-fertility rate than parent 7182. In addition, compared with 7182, H-24-3-1-5-19-1 had a much lower plant height while the other agronomic traits were relatively similar. The two new lines are valuable germplasm materials for breeding FHB resistance in wheat.

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