Type III Secretion System of Bradyrhizobium sp. SUTN9-2 Obstructs Symbiosis with Lotus spp.

Shun Hashimoto; Kohki Goto; Pongdet Pyromyou; Pongpan Songwattana; Teerana Greetatorn; Panlada Tittabutr; Nantakorn Boonkerd; Neung Teaumroong; Toshiki Uchiumi

doi:10.1264/jsme2.ME20041

Short Communication

Type III Secretion System of Bradyrhizobium sp. SUTN9-2 Obstructs Symbiosis with Lotus spp.

Shun Hashimoto, Kohki Goto, Pongdet Pyromyou, Pongpan Songwattana, Teerana Greetatorn, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, Toshiki Uchiumi

Author information

Shun Hashimoto
Graduate School of Science and Engineering, Kagoshima University
Kohki Goto
Graduate School of Science and Engineering, Kagoshima University
Pongdet Pyromyou
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Pongpan Songwattana
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Teerana Greetatorn
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Panlada Tittabutr
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Nantakorn Boonkerd
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Neung Teaumroong
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Toshiki Uchiumi
Graduate School of Science and Engineering, Kagoshima University

Keywords: symbiosis, Bradyrhizobium, Lotus spp., type III secretion system, effector protein

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Supplementary material

2020 Volume 35 Issue 3 Article ID: ME20041

DOI https://doi.org/10.1264/jsme2.ME20041

Details

Abstract

The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotus burttii. A mutant of SUTN9-2 that is unable to secrete effector proteins showed better nodulation and plant growth promotion than wild-type SUTN9-2 when paired with these Lotus spp. We propose that SUTN9-2 is a useful strain for understanding the mechanisms by which effector proteins obstruct symbiosis between Bradyrhizobium and Lotus spp.

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