Distribution of N-Acylhomoserine Lactone-Producing Fluorescent Pseudomonads in the Phyllosphere and Rhizosphere of Potato (Solanum tuberosum L.)

Nobutaka Someya; Tomohiro Morohoshi; Nobuya Okano; Eiko Otsu; Kazuei Usuki; Mitsuru Sayama; Hiroyuki Sekiguchi; Tsukasa Ikeda; Shigeki Ishida

doi:10.1264/jsme2.ME09155

Regular Papers

Distribution of N-Acylhomoserine Lactone-Producing Fluorescent Pseudomonads in the Phyllosphere and Rhizosphere of Potato (Solanum tuberosum L.)

Nobutaka Someya, Tomohiro Morohoshi, Nobuya Okano, Eiko Otsu, Kazuei Usuki, Mitsuru Sayama, Hiroyuki Sekiguchi, Tsukasa Ikeda, Shigeki Ishida

Author information

Nobutaka Someya
National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)
Tomohiro Morohoshi
Department of Applied Chemistry, Utsunomiya University
Nobuya Okano
Department of Applied Chemistry, Utsunomiya University
Eiko Otsu
National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)
Kazuei Usuki
National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)
Mitsuru Sayama
National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)
Hiroyuki Sekiguchi
National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)
Tsukasa Ikeda
Department of Applied Chemistry, Utsunomiya University
Shigeki Ishida
National Agricultural Research Center for Hokkaido Region (NARCH), National Agriculture and Food Research Organization (NARO)

Keywords: fluorescent pseudomonad, quorum sensing, N-acylhomoserine lactone, Solanum tuberosum L., 16S rRNA gene

JOURNAL FREE ACCESS

2009 Volume 24 Issue 4 Pages 305-314

DOI https://doi.org/10.1264/jsme2.ME09155

View "Advance Publication" version (October 30, 2009).

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Abstract

Four hundred and fifty nine isolates of fluorescent pseudomonads were obtained from the leaves and roots of potato plants. Of these, 20 leaf isolates and 28 root isolates induced violacein production in two N-acylhomoserine lactone (AHL)-reporter strains—Chromobacterium violaceum CV026 and VIR24. VIR24 is a new reporter strain for long N-acyl-chain-homoserine lactones, which can not be detected by CV026. Thin-layer chromatography revealed that the isolates produced multiple AHL molecules. We compared the 16S rRNA gene sequences of these isolates with sequences from a known database, and examined phylogenetic relationships. The AHL-producing isolates generally separated into three groups. Group I was mostly composed of leaf isolates, and group III, root isolates. Group II comprised both leaf and root isolates. There was a correlation between the phylogenetic cluster and the AHL molecules produced and some phenotypic characteristics. Our study confirmed that AHL-producing fluorescent pseudomonads could be distinguished in the phyllosphere and rhizosphere of potato plants.

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