Evaluation of L929 fibroblast attachment and proliferation on Arg-Gly-Asp-Ser (RGDS)-immobilized chitosan in serum-containing/serum-free cultures
Section snippets
Materials
Chitosan derived from crab shell (> 85% deacetylation; cat. no. C 3646) and RGDS with a FW of 433.4 were purchased from Sigma (Munich, Germany). Photochemical cross-linker sulfosuccinimidyl 6 (4′-azido-2′-nitrophenyl-amino) hexanoate (sulfo-SANPAH) with a FW of 492.4 was obtained from Pierce (Rockford, IL, USA). Phosphate-buffered saline (PBS) tablets were purchased from Sigma. Tissue culture clusters, flasks and plates were purchased from Nunc (Hannover, Germany). Dulbecco’s modified Eagle’s
Biomodification of chitosan membranes
The RGDS tetrapeptide is known to cause a distinct increase in cell attachment compared to the tripeptide sequence (RGD), confirming that peptides with higher integrin affinity cause higher cell attachment activity (6). In this study, RGDS was immobilized on the surface of chitosan membranes to construct a bioactive material for stimulated fibroblast attachment and growth. Photochemical immobilization technique which does not require specific activated groups on the surfaces (21, 22, 24) was
Acknowledgments
This study was financially supported by Grant No. 03K120570 from the Turkish Republic Prime Ministry State Planning Organization and by FIS-CNR (Rome) and FIRB RBNE01458S grants. A. Karakeçili acknowledges the grant provided by the Consorzio Interuniversitario per i Sistemi a Grande Interfaccia (CSGI, Florence, Italy) for the period spent at LAMSUN at University of Catania.
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