Short-Term Hormone Release from Adult Female Rat Hypothalamic and Pituitary Explants is not Altered by 2,3,7,8-Tetrachlorodibenzo-p-dioxin

Amanda Louise TREWIN; Michael John WOLLER; Barbara Ann Brown WIMPEE; Lisa Kay CONLEY; Monika Gabriella BALDRIDGE; Reinhold J. HUTZ

doi:10.1262/jrd.18101

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Short-Term Hormone Release from Adult Female Rat Hypothalamic and Pituitary Explants is not Altered by 2,3,7,8-Tetrachlorodibenzo-p-dioxin

Amanda Louise TREWIN, Michael John WOLLER, Barbara Ann Brown WIMPEE, Lisa Kay CONLEY, Monika Gabriella BALDRIDGE, Reinhold J. HUTZ

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Keywords: Hypothalamus, Pituitary, 2, 3, 7,8-Tetrachlorodibenzo-p-dioxin (TCDD), GnRH, Gonadotropins

JOURNAL FREE ACCESS

2007 Volume 53 Issue 4 Pages 765-775

DOI https://doi.org/10.1262/jrd.18101

View "Advance Publication" version (February 27, 2007).

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Abstract

2, 3, 7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has adverse effects on reproduction, in part due to direct actions at the ovary. It is unclear whether effects are further mediated by glands that regulate ovarian function. We investigated whether effects of TCDD are mediated via the hypothalamic-pituitary axis. Hypothalamic and pituitary tissues were cultured in medium with and without TCDD. TCDD did not alter GnRH release from hypothalamic samples. It continued to be pulsatile with no differences in the average peak frequency, average peak amplitude, or baseline GnRH release. TCDD did not alter GnRH-induced release of gonadotropins from pituitary samples. There were no differences in average peak amplitude or baseline release. AhR, ARNT or ERα mRNA copy numbers in cultured pituitaries were not affected by TCDD. Our data suggest that TCDD effects on ovarian function are not mediated through the hypothalamic or pituitary release parameters tested in this study.

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