1,25-Dihydroxyvitamin D3 Suppresses Gene Expression of Eukaryotic Translation Initiation Factor 2 in Human Promyelocytic Leukemia HL-60 Cells

Takuji Suzuki; Yu Koyama; Hiroyasu Ichikawa; Koji Tsushima; Kouichi Abe; Sumio Hayakawa; Ryoko Kuruto-Niwa; Ryushi Nozawa; Mamoru Isemura

doi:10.1247/csf.30.1

1,25-Dihydroxyvitamin D₃ Suppresses Gene Expression of Eukaryotic Translation Initiation Factor 2 in Human Promyelocytic Leukemia HL-60 Cells

Takuji Suzuki, Yu Koyama, Hiroyasu Ichikawa, Koji Tsushima, Kouichi Abe, Sumio Hayakawa, Ryoko Kuruto-Niwa, Ryushi Nozawa, Mamoru Isemura

Author information

Takuji Suzuki
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka
Yu Koyama
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka
Hiroyasu Ichikawa
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka
Koji Tsushima
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka
Kouichi Abe
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka
Sumio Hayakawa
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka
Ryoko Kuruto-Niwa
Microbiology and Host Defense, School of Food and Nutritional Sciences, University of Shizuoka
Ryushi Nozawa
Microbiology and Host Defense, School of Food and Nutritional Sciences, University of Shizuoka
Mamoru Isemura
Department of Biochemistry, School of Food and Nutritional Sciences, and COE for 21^st Century, University of Shizuoka

Keywords: vitamin D₃, differentiation, gene expression, initiation factor, HL-60

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2005 Volume 30 Issue 1 Pages 1-6

DOI https://doi.org/10.1247/csf.30.1

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Abstract

The physiologically active metabolite of vitamin D₃, 1α,25-dihydroxyvitamin D₃ (DVD), is a potent inducer of cell differentiation in human myeloid leukemia cells. In the present study, we examined changes in gene expression during DVD-induced cell differentiation of promyelocytic HL-60 cells employing a DNA microarray technique. The results identified 7 up-regulated and 9 down-regulated genes with a change greater than 1.5-fold after the DVD-treatment for both 2 and 6 days. Seven of these genes were further examined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that findings obtained from the DNA microarray analysis and RT-PCR are generally comparable with each other. Gene expression of the subunits of eukaryotic translation initiation factor 2 was then examined by methods including RT-PCR and real-time PCR. The results indicated the suppression of these genes, suggesting a linkage to differentiation-associated growth inhibition of these cells.

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