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Preparation, Characterization and Evaluation of Marsupsin–Phospholipid Complex

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Abstract

The aim of this research was to formulate Marsupsin–phospholipid complex (M–P Complex) in attempt to increase the bioavailability of marsupsin and to characterize this new formulation along with its evaluation. Marsupsin–phospholipid complex was formulated by mechanical dispersion method. In this new formulation, complex formation was confirmed by carrying out transmission electron microscopy (TEM), IR, 1H-NMR and RP-HPLC analysis. TEM showed M–P Complex diameter range of 0.05–0.5 μm. The entrapment efficiency of M–P Complex was found to be 44%. In vitro release study revealed its first order release profile. Mean blood serum concentration vs time curve of marsupsin was of first order after oral administration of M–P Complex in albino rabbits which clearly showed remarkably increased bioavailability of M–P Complex than standardized marsupsin. The average value of C max and T max of M–P Complex were found to be 3.02 mg/ml and 10.2 h, respectively. Hence the findings demonstrate that complexing marsupsin with phospholipids results in better oral bioavailability and improved biological response than free form of standardized marsupsin.

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Acknowledgments

The authors are obliged to Dr. V. B. Gupta, Director B. R. Nahata college of Pharmacy for his valuable support during this research. Authors are also thankful to CDRI Lucknow and All India institute of medical sciences (AIIMS), New Delhi for support in analytical portion.

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Authors and Affiliations

  1. Department of Pharmacognosy and Phytochemistry, B. R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur, 458001, India

    Mukesh S. Sikarwar, Amit K. Jain & S. D. Parial

  2. Department of Dravyaguna, D. G. M. Ayurvedic Medical College, Kalashpur Road, Gadag, Karnataka, India

    Shalini Sharma

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  1. Mukesh S. Sikarwar
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  2. Shalini Sharma
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Correspondence to Mukesh S. Sikarwar.

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Sikarwar, M.S., Sharma, S., Jain, A.K. et al. Preparation, Characterization and Evaluation of Marsupsin–Phospholipid Complex. AAPS PharmSciTech 9, 129–137 (2008). https://doi.org/10.1208/s12249-007-9020-x

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