Journal of Lipid Research
Research ArticlesA comparative study on fluorescent cholesterol analogs as versatile cellular reporters[S]
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This work was supported by funding from the Wolfson Foundation, the Medical Research Council (MRC, Grants MC_UU_12010/unit programmes G0902418 and MC_UU_12025), MRC/BBSRC/ESPRC (Grant MR/K01577X/1), and the Wellcome Trust (Grant 104924/14/Z/14). E.S. was supported by EMBO Long Term and Marie Curie Intra-European Fellowships (MEMBRANE DYNAMICS). M.P.C. was supported by the Alfred Benzon Foundation research stipend. D.W. acknowledges support from the Villum and Novo Nordisk Foundation. A.C. and F.P. acknowledge the European Union Seventh Framework Programme (FP7 2007–2013, “Sphingonet”, grant agreement 289278). F.P. is a Royal Society Wolfson Research Merit Award holder.
- Chol
cholesterol
- cpm
counts per molecule
- CTL
cholestatrienol
- CW
continuous-wave
- DChol
6-dansyl-cholesterol
- DHE
dehydroergosterol
- DOPC
1,2-dioleoyl-sn-glycero-3-phosphocholine
- ERC
endocytic recycling compartment
- FCS
fluorescence correlation spectroscopy
- GPMV
giant plasma membrane vesicle
- GUV
giant unilamellar vesicle
- Ld
liquid disordered
- Lo
liquid ordered
- %Lo
liquid ordered partitioning coefficient
- NBD
7-nitrobenz-2-oxa-1,3-diazol-4-yl
- NPC
Niemann-Pick type C
- PCC
Pearson correlation coefficient
- SLB
supported lipid bilayer
- STED
stimulated emission depletion
- TF
TopFluor
- TF-Chol
TopFluor cholesterol
Abbreviations:
- [S]
The online version of this article (available at http://www.jlr.org) contains a supplement.