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Increased Expression of KISSI and KISSI Receptor in Human Granulosa Lutein Cells—Potential Pathogenesis of Polycystic Ovary Syndrome

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Abstract

Kisspeptins are a family of neuropeptides that are essential for fertility. Recent experimental data suggest a putative role of kisspeptin signaling in the direct control of ovarian function. To explore the expression of KISS1 and KISS1 receptor (KISS1R) in human granulosa lutein cells and the potential role of KISS1/KISS1R system in the pathogenesis of polycystic ovary syndrome (PCOS), we measured the concentration of KISS1 in follicular fluid, the expression of KISS1 and KISS1R in granulosa lutein cells, and the circulating hormones. The expression levels of KISS1 and KISS1R were significantly upregulated in human granulosa lutein cells obtained from women with PCOS. The expression levels of KISS1 in human granulosa lutein cells highly correlated with those of KISS1R in non-PCOS patients, but not in patients with PCOS, most likely due to the divergent expression patterns in women with PCOS. Additionally, the expression levels of KISS1 highly correlated with the serum levels of anti-Mullerian hormone (AMH). The expression levels of KISS1 and KISS1R, as well as the follicular fluid levels of KISS1, were not significantly different between the pregnant and nonpregnant patients in both PCOS and non-PCOS groups. In conclusion, the increased expression of KISS1 and KISS1R in human granulosa lutein cells may contribute to the pathogenesis of PCOS. The expression levels of KISS1 highly correlated with the serum levels of AMH. The KISS1 and KISS1R system in the ovary may not have a remarkable role in predicting the in vitro fertilization (IVF) outcome.

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Acknowledgments

The authors appreciate the help in statistical analysis from Professor Zhao in Peking University Third Hospital.

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Authors and Affiliations

  1. Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China

    Kai-Lun Hu, Hongcui Zhao, Zheying Min, Yilei He, Tianjie Li, Xiumei Zhen, Yun Ren, Yang Yu & Rong Li

  2. National Clinical Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China

    Hongcui Zhao, Xiumei Zhen, Yun Ren & Yang Yu

  3. Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China

    Zheying Min

  4. Department of Obstetrics and Gynaecology, University of British Columbia and British Columbia Children’s Hospital Research Institute, Vancouver, British Columbia, Canada

    Hsun-Ming Chang

Authors
  1. Kai-Lun Hu
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  2. Hongcui Zhao
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  3. Zheying Min
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  4. Yilei He
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  5. Tianjie Li
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  7. Yun Ren
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  8. Hsun-Ming Chang
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  9. Yang Yu
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  10. Rong Li
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Corresponding author

Correspondence to Rong Li.

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Hu, KL., Zhao, H., Min, Z. et al. Increased Expression of KISSI and KISSI Receptor in Human Granulosa Lutein Cells—Potential Pathogenesis of Polycystic Ovary Syndrome. Reprod. Sci. 26, 1429–1438 (2019). https://doi.org/10.1177/1933719118818899

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