Skip to main content

Advertisement

SpringerLink
Log in

Placental-Derived Mesenchyme Influences Chorionic Gonadotropin and Progesterone Secretion of Human Embryonic Stem Cell-Derived Trophoblasts

  • Article
  • Published:
Reproductive Sciences Aims and scope Submit manuscript

Abstract

Studies of early placental development in humans are difficult because of limitations on experimental material availability from the perimplantation period. We used a coculture system to determine the effects of various effector cell types on trophoblast differentiation. Enhanced green fluorescent protein (EGFP)-expressing H1 human embryonic stem cells were used in co-suspension with human term placental fibroblasts (TPFs) and dermal fibroblasts (CI2F) to form combination embryoid bodies (EBs), with the goal of recapitulating placental morphogenesis through incorporation of placental mesenchymal cells. Overall, the results demonstrated that when using mesenchymal cells for EB preparation from term placentas (TPF), combination EB-derived trophoblasts secrete higher levels of human chorionic gonadotropin (hCG) and progesterone compared to EBs made without effector cells, whereas there was no effect of dermal fibroblasts. This is due to the secretory activity of the EB-derived trophoblasts and not due to the number of differentiated trophoblasts per EB, demonstrating that nontrophoblast cells of the placenta can influence trophoblast endocrine activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Benirschke K, Kaufman P, Baergen RN. Pathology of the Human Placenta. 5th ed. New York, NY: Springer-Verlag; 1999.

    Google Scholar 

  2. Vicovac L, Aplin JD. Epithelial-mesenchymal transition during trophoblast differentiation. Acta Anat (Basel). 1996;156(3): 202–216.

    Article  CAS  Google Scholar 

  3. Damsky CH, Fisher SJ. Trophoblast pseudo-vasculogenesis: faking it with endothelial adhesion receptors. Curr Opin Cell Biol. 1998;10(5):660–666.

    Article  CAS  PubMed  Google Scholar 

  4. Red-Horse K, Zhou Y, Genbacev O, et al. Trophoblast differentiation during embryo implantation and formation of the maternalfetal interface. J Clin Invest. 2004;114(6):744–754.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Kaufmann P, Mayhew TM, Charnock-Jones DS. Aspects of human fetoplacental vasculogenesis and angiogenesis. III. Changes in complicated pregnancies. Placenta. 2004;25(2–3):114–126.

    Article  CAS  PubMed  Google Scholar 

  6. Golos TG, Pollastrinin LM, Gerami-Naini B. Human embryonic stem cells as a model for trophoblast differentiation. Semin Reprod Med. 2006;24(5):314–321.

    Article  CAS  PubMed  Google Scholar 

  7. Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282(5391):1145–1147.

    Article  CAS  PubMed  Google Scholar 

  8. Xu RH, Chen X, Li DS, et al. BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat Biotechnol. 2002;20(12):1261–1264.

    Article  CAS  PubMed  Google Scholar 

  9. Gerami-Naini B, Dovzhenko OV, Durning M, Wegner FH, Thomson JA, Golos TG. Trophoblast differentiation in embryoid bodies derived from human embryonic stem cells as a model for trophoblast differentiation. Endocrinology. 2004;145(4): 1517–1524.

    Article  CAS  PubMed  Google Scholar 

  10. Liu YP, Dovzhenko OV, Garthwaite MA, et al. Maintenance of pluripotency in human embryonic stem cells stably overexpressing enhanced green fluorescent protein. Stem Cells Dev. 2004;13(6):636–645.

    Article  CAS  PubMed  Google Scholar 

  11. Xu C, Inokuma MS, Denham J, et al. Feeder-free growth of undifferentiated human embryonic stem cells. Nat Biotechnol. 2001;19(10):971–974.

    Article  CAS  PubMed  Google Scholar 

  12. Bondarenko GI, Burleigh DW, Durning M, Breburda EE, Grendell RL, Golos TG. Passive immunization against the MHC class I molecule Mamu-AG disrupts rhesus placental development and endometrial responses. J Immunol. 2007;179(12): 8042–8050.

    Article  CAS  PubMed  Google Scholar 

  13. Thomson JA, Kalishman J, Golos TG, et al. Isolation of a primate embryonic stem cell line. Proc Natl Acad Sci. U S A. 1995;92(17): 7844–7848.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Peiffer I, Belhomme D, Barbet R, et al. Simultaneous differentiation of endothelial and trophoblastic cells derived from human embryonic stem cells. Stem Cells Dev. 2007;16(3): 393–402.

    Article  CAS  PubMed  Google Scholar 

  15. Soares MJ, Wolfe MW. Human embryonic stem cells assemble and fulfill their developmental destiny. Endocrinology. 2004;145(4):1514–1516.

    Article  CAS  PubMed  Google Scholar 

  16. Schulz LC, Ezashi T, Das P, Westfall SD, Livingston KA, Roberts RM. Human embryonic stem cells as models for trophoblast differentiation. Placenta. 2008;29(suppl A):S10–S16.

    Article  PubMed  Google Scholar 

  17. Harun R, Ruban L, Matin M, et al. Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capacity to mimic invasive implantation events. Hum Reprod. 2006;21(6): 1349–1358.

    Article  CAS  PubMed  Google Scholar 

  18. Genbacev O, Krtolica A, Zdravkovic T, et al. Serum-free derivation of human embryonic stem cell lines on human placental fibroblast feeders. Fertil Steril. 2005;83(5):1517–1529.

    Article  PubMed  Google Scholar 

  19. Simón C, Escobedo C, Valbuena D, et al. First derivation in Spain of human embryonic stem cell lines: use of long-term cryopre-served embryos and animal-free conditions. Fertil Steril. 2005;83(1):246–249.

    Article  PubMed  Google Scholar 

  20. Xu RH, Peck RM, Li DS, Feng X, Ludwig T, Thomson JA. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods. 2005;2(3):185–190.

    Article  CAS  PubMed  Google Scholar 

  21. Jones DL, Wagers AJ. No place like home: anatomy and function of the stem cell niche. Nat Rev Mol Cell Biol. 2008;9(1): 11–21.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Wisconsin National Primate Research Center, University of Wisconsin-Madison, 1223 Capitol Court, 53715, Madison, WI, USA

    Maria Giakoumopoulos PhD, Leah M. Siegfried MS, Svetlana V. Dambaeva MD, PhD, Mark A. Garthwaite BS, M. Clay Glennon BS & Thaddeus G. Golos PhD

  2. Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA

    Maria Giakoumopoulos PhD, Leah M. Siegfried MS, Mark A. Garthwaite BS & Thaddeus G. Golos PhD

  3. Department of Comparative Biosciences, University of Wisconsin-Madison, Madison, WI, USA

    Svetlana V. Dambaeva MD, PhD & Thaddeus G. Golos PhD

Authors
  1. Maria Giakoumopoulos PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leah M. Siegfried MS
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Svetlana V. Dambaeva MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark A. Garthwaite BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Clay Glennon BS
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Thaddeus G. Golos PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Thaddeus G. Golos PhD.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Giakoumopoulos, M., Siegfried, L.M., Dambaeva, S.V. et al. Placental-Derived Mesenchyme Influences Chorionic Gonadotropin and Progesterone Secretion of Human Embryonic Stem Cell-Derived Trophoblasts. Reprod. Sci. 17, 798–808 (2010). https://doi.org/10.1177/1933719110371853

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1177/1933719110371853

Keywords

Search

Navigation