Skip to main content

Advertisement

SpringerLink
Log in

Elevated Plasma Stromal Cell-derived Factor 1 Protein and its Gene Polymorphism in Patients With Pelvic Inflammatory Disease

  • Published:
Reproductive Sciences Aims and scope Submit manuscript

Abstract

The objective was to compare the expression of plasma stromal cell-derived factor 1 and the gene polymorphism in patients with pelvic inflammatory disease and healthy controls. The enzyme-linked immunosorbent assay and polymerase chain reaction—restriction fragment length polymorphism were, respectively, used to measure the plasma stromal cell-derived factor 1a level and stromal cell-derived factor 1 polymorphism in 50 healthy controls and in 44 patients with pelvic inflammatory disease before and after they received routine treatment protocols. The level of plasma stromal cell-derived factor 1a was elevated in patients with pelvic inflammatory disease compared to normal controls and decreased significantly after treatment. There were significant correlations between plasma stromal cell-derived factor 1 a level and neutrophil count as well as between stromal cell-derived factor 1 a level and white blood cell count in patients with pelvic inflammatory disease. There was no significantly different distribution of stromal cell-derived factor 1 genotypes between patients with pelvic inflammatory disease and normal controls. Patients with pelvic inflammatory disease having stromal cell-derived factor 1–3’ A allele were associated with significantly elevated plasma stromal cell-derived factor 1 a concentration compared to patients with pelvic inflammatory disease having G/G homozygous alleles (P < .02). In normal controls, there was no significant difference in the plasma stromal cell-derived factor 1 level between individuals with and without stromal cell-derived factor 1–3’A allele. When the cutoff level of plasma stromal cell-derived factor 1a level was determined to be 2192 pg/mL based on receiver-operating characteristic curve, the sensitivity, specificity, positive predictive value, and negative predictive value as well as accuracy were 77.3%, 88.0%, 85.0%, 81.5%, and 83.0%. In conclusion, when the cutoff level was determined to be 2192 pg/mL, plasma stromal cell-derived factor 1 a level can be used to predict pelvic inflammatory disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Borish LC, Steinke JW. Cytokines and chemokines. J Allergy Clin Immunol. 2003;111:S460–S475.

    Article  CAS  Google Scholar 

  2. Crazzolara R, Kreczy A, Mann G, et al. High expression of the chemokine receptor CXCR4 predicts extramedullary organ infiltration in childhood acute lymphoblastic leukaemia. Br J Haematol. 2001;115:545–553.

    Article  CAS  Google Scholar 

  3. Shimoda K, Begum NA, Shibuta K, et al. Interleukin–8 and hIRH (SDF1-alpha/PBSF) mRNA expression and histologi-cal activity index in patients with chronic hepatitis C. Hepatol-ogy. 1998;28:108–115.

  4. Buckley CD, Amft N, Bradfield PF, et al. Persistent induction of the chemokine receptor CXCR4 by TGF-beta 1 on synovial T cells contributes to their accumulation within the rheumatoid synovium. J Immunol. 2000;165:3423–3429.

    Article  CAS  Google Scholar 

  5. Sutton A, Friand V, Brule-Donneger S, et al. Stromal cell-derived factor 1/chemokine (C-X-C motif) ligand 12 stimulates human hepatoma cell growth, migration, and invasion. Mol Cancer Res. 2007;5:21–33.

    Article  CAS  Google Scholar 

  6. Gorter DJ, Reijmers RM, Beuling EA, et al. The small GTPase Ral mediates SDF-1-induced migration of B cells and multiple myeloma cells. Blood. 2008;111:3364–3372.

    Article  Google Scholar 

  7. Kerfoot SM, Andonegui G, Bonder CS, Liu L. Exogenous stromal cell-derived factor–1 induces modest leukocyte recruitment in vivo. Am J Physiol Heart Circ Physiol. 2008;294:H2524–H2534.

    Article  CAS  Google Scholar 

  8. Abi-Younes S, Sauty A, Mach F, Sukhova GK, Libby P, Luster AD. The stromal cell-derived factor–1 chemokine is a potent platelet agonist highly expressed in atherosclerotic plaques. Circ Res. 2000;86:131–138.

    Article  CAS  Google Scholar 

  9. Burgoyne CH, Field SL, Brown AK, et al. Abnormal T-cell differentiation persists in rheumatoid arthritis patients in clinical remission and predicts relapse. Ann Rheum Dis. 2008;67:750–757.

    Article  CAS  Google Scholar 

  10. Leone AM, Rutella S, Bonanno G, et al. Endogenous G-CSF and CD34+ cell mobilization after acute myocardial infarction. Int J Cardiol. 2006;111:202–208.

    Article  Google Scholar 

  11. Shirozu M, Nakano T, Inazawa J, et al. Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene. Genomics. 1995;28:495–500.

    Article  CAS  Google Scholar 

  12. Apostolakis S, Baritaki S, Kochiadakis GE, Igoumenidis NE, Panutsopulos D, Spandidos DA. Effects of polymorphisms in chemokine ligands and receptors on susceptibility to coronary artery disease. Thromb Res. 2007;119:63–71.

    Article  CAS  Google Scholar 

  13. Winkler C, Modi W, Smith MW, et al. Genetic restriction of AIDS pathogenesis by an SDF-1 chemokine gene variant. ALIVE Study, Hemophilia Growth and Development Study (HGDS), Multicenter AIDS Cohort Study (MACS), Multicenter Hemophilia Cohort Study (MHCS), San Francisco City Cohort (SFCC). Science. 1998;279:389–393.

    Article  CAS  Google Scholar 

  14. Soriano A, Martinez C, Garcia F, et al. Plasma stromal cell-derived factor (SDF)–1 levels, SDF1–3’A genotype, and expression of CXCR4 on T lymphocytes: their impact on resistance to human immunodeficiency virus type 1 infection and its progression. J Infect Dis. 2002;186:922–931.

    Article  CAS  Google Scholar 

  15. Brambilla A, Villa C, Rizzardi G, et al. Shorter survival of SDF1–3’A/3’A homozygotes linked to CD4+ T cell decrease in advanced human immunodeficiency virus type 1 infection. J Infect Dis. 2000;182:311–315.

    Article  CAS  Google Scholar 

  16. Oliveira CE, Cavassin GG, Perim Ade L, et al. Stromal cell-derived factor–1 chemokine gene variant in blood donors and chronic myelogenous leukemia patients. J Clin Lab Anal. 2007;21:49–54.

    Article  Google Scholar 

  17. Coll B, Parra S, Alonso-Villaverde C, et al. The role of immunity and inflammation in the progression of atherosclerosis in patients with HIV infection. Stroke. 2007;38:2477–2484.

    Article  Google Scholar 

  18. Corbeil LB, Shively JN, Duncan JR, Schurig GG, Winter AJ. Bovine venereal vibriosis. Ultrastructure of endometrial inflammatory lesions. Lab Invest. 1975;33:187–192.

    CAS  PubMed  Google Scholar 

  19. Tsai HT, Wang PH, Tee YT, Lin LY, Hsieh YS, Yang SF. Imbalanced serum concentration between cathepsin B and cystatin C in patients with pelvic inflammatory disease. Fertil Steril. IN PRESS.

  20. Dayan L. Pelvic inflammatory disease. Aust Fam Physician. 2006;35:858–862.

    PubMed  Google Scholar 

  21. Gray-Swain MR, Peipert JF. Pelvic inflammatory disease in adolescents. Curr Opin Obstet Gynecol. 2006;18:503–510.

    Article  Google Scholar 

  22. Hadgu A, Westrom L, Brooks CA, Reynolds GH, Thompson SE. Predicting acute pelvic inflammatory disease: a multivariate analysis. Am J Obstet Gynecol. 1986;155:954–960.

    Article  CAS  Google Scholar 

  23. Angerman NS, Evans MI, Moravec WD, Schumacher GF, Hajj SN. C-reactive protein in the evaluation of antibiotic therapy for pelvic infection. J Reprod Med. 1980;25:63–66.

    CAS  PubMed  Google Scholar 

  24. Katsuta T, Lim C, Shimoda K, et al. Interleukin–8 and SDF1-alpha mRNA expression in colonic biopsies from patients with inflammatory bowel disease. Am J Gastroenterol. 2000;95:3157–3164.

    CAS  PubMed  Google Scholar 

  25. Kinnunen A, Molander P, Morrison R, et al. Chlamydial heat shock protein 60—specific T cells in inflamed salpingeal tissue. Fertil Steril. 2002;77:162–166.

    Article  Google Scholar 

  26. Sukhikh GT, Kasabulatov NM, Van’ko LV, Ordzhonikidze NV, Veryasov VN, Danelyan SZh. Ratio between the number of Th1 and Th2 lymphocytes in the peripheral blood and concentration of proinflammatory cytokines in lochia of women with postpartum endometritis. Bull Exp Biol Med. 2005;140:672–674.

    Article  CAS  Google Scholar 

  27. Chen KS, Wang PH, Yang SF, et al. Significant elevation of a Th2 cytokine, interleukin–10, in pelvic inflammatory disease. Clin Chem Lab Med. 2008;46:1609–1616.

    Article  CAS  Google Scholar 

  28. Lee SA, Tsai HT, Ou HC, et al. Plasma interleukin–1beta, –6, –8 and tumor necrosis factor-alpha as highly informative markers of pelvic inflammatory disease. Clin Chem Lab Med. 2008;46:997–1003.

    CAS  PubMed  Google Scholar 

  29. Kimura R, Nishioka T, Ishida T. The SDF1-G801A polymorphism is not associated with SDF1 gene expression in Epstein-Barr virus-transformed lymphoblastoid cells. Genes Immun. 2003;4:356–61.

    Article  CAS  Google Scholar 

  30. Sei S, O’Neill DP, Stewart SK, et al. Increased level of stromal cell-derived factor–1 mRNA in peripheral blood mononuc-lear cells from children with AIDS-related lymphoma. Cancer Res. 2001;61:5028–5037.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Nursing, College of Nursing, Chung Shan Medical University, Taichung, Taiwan

    Hsiu-Ting Tsai PhD & Hsiu-Chen Tsai PhD

  2. School of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Yi-Torng Tee MD, PhD & Po-Hui Wang MD, PhD

  3. Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung, Taiwan

    Yi-Torng Tee MD, PhD & Po-Hui Wang MD, PhD

  4. Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan

    Yi-Hsien Hsieh PhD

  5. School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Chien-Kuo N. Road, 110, Taichung, Taiwan

    Hui-Ling Chiou PhD & Shun-Fa Yang PhD

  6. Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan

    Hui-Ling Chiou PhD, Chiao-Wen Lin MS & Shun-Fa Yang PhD

  7. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan

    Shun-Fa Yang PhD

Authors
  1. Hsiu-Ting Tsai PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yi-Torng Tee MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi-Hsien Hsieh PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hui-Ling Chiou PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chiao-Wen Lin MS
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hsiu-Chen Tsai PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Po-Hui Wang MD, PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shun-Fa Yang PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shun-Fa Yang PhD.

Additional information

No potential conflicts of interest were disclosed.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tsai, HT., Tee, YT., Hsieh, YH. et al. Elevated Plasma Stromal Cell-derived Factor 1 Protein and its Gene Polymorphism in Patients With Pelvic Inflammatory Disease. Reprod. Sci. 16, 610–617 (2009). https://doi.org/10.1177/1933719109332829

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1177/1933719109332829

Key words

Search

Navigation