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Combination of CCR1 mRNA, MCP1, and CA125 Measurements in Peripheral Blood as a Diagnostic Test for Endometriosis

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Abstract

This study investigated the possible use of CCR1 mRNA measurement in peripheral blood leukocytes in combination with measurements of monocyte chemotactic protein-1 (MCP-1) and CA125 protein in serum as a diagnostic test for endometriosis. The expression of CCR1 mRNA in peripheral blood leukocytes was measured by quantitative real-time polymerase chain reaction. MCP-1 and CA125 levels in serum were determined by ELISA and ECLIA.The ratio of CCR1/HPRT mRNA in peripheral blood of patients with endometriosis and adenomyosis was significantly elevated compared with women without endometriosis. Additionally, serum levels of MCP-1 and CA125 were significantly higher in patients with endometriosis. This method showed a sensitivity of 92.2%, a specificity of 81.6%, a negative predictive value of 83.3%, a positive predictive value of 92.3%, a likelihood ratio of a positive test result of 5.017, and a likelihood ratio of a negative test result of 0.096 to predict the presence or absence of endometriosis. The results imply the potential use of CCR1 mRNA, MCP-1, and CA125 protein measurements for the diagnosis or exclusion of endometriosis.

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, University of Schleswig-Holstein, Ratzeburgerallee 160, Luebeck, Germany

    Admir Agic MD, Schima Djalali MD, Klaus Diedrich MD, PhD & Daniela Hornung MD, PhD

  2. Department of Gynecology and Obstetrics, Technical University of Aachen, Aachen, Germany

    Monika M. Wolfler MD

  3. Praxisklinik für Fertilität, Am Gendarmenmarkt, Berlin, Germany

    Gulden Halis MD

Authors
  1. Admir Agic MD
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  2. Schima Djalali MD
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  3. Monika M. Wolfler MD
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  4. Gulden Halis MD
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  5. Klaus Diedrich MD, PhD
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  6. Daniela Hornung MD, PhD
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Correspondence to Daniela Hornung MD, PhD.

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Agic, A., Djalali, S., Wolfler, M.M. et al. Combination of CCR1 mRNA, MCP1, and CA125 Measurements in Peripheral Blood as a Diagnostic Test for Endometriosis. Reprod. Sci. 15, 906–911 (2008). https://doi.org/10.1177/1933719108318598

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  • DOI: https://doi.org/10.1177/1933719108318598

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