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Acetylcholinesterase Biosensor for Carbaryl Detection Based on Nano-Porous Pseudo Carbon Paste Electrode

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A highly selective acetylcholinesterase (AChE) biosensor based on self-made ultrasensitive nano-porous pseudo carbon paste electrode (nano-PPCPE) modified with chitosan (CS), gold nanoparticles (GNPs), Nafion (NF) and AChE has been successfully developed, and used to detect carbaryl pesticides. The inhibition of AChE increased along with the increase in concentration of carbaryl, and the linear ranges were from 1.0 × 10–7 g/mL to 5.0 × 10–10 g/mL and 1.0 × 10–10 g/mL to 1.0 × 10–13 g/mL with the detection limit as low as 5.0 × 10–14 g/mL. The proposed biosensor exhibited good stability, high sensitivity and selectivity towards carbaryl.

Keywords: ACETYLCHOLINESTERASE; CARBARYL; DETECTION; NANO-POROUS PSEUDO CARBON PASTE ELECTRODE (NANO-PPCPE)

Document Type: Short Communication

Publication date: 01 September 2016

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  • Nanoscience and Nanotechnology Letters (NNL) is a multidisciplinary peer-reviewed journal consolidating nanoscale research activities in all disciplines of science, engineering and medicine into a single and unique reference source. NNL provides the means for scientists, engineers, medical experts and technocrats to publish original short research articles as communications/letters of important new scientific and technological findings, encompassing the fundamental and applied research in all disciplines of the physical sciences, engineering and medicine.
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