Abstract
We revised the taxonomic status of the putative Acomys spinosissimus complex based on the comparative study of specimen collections from Tanzania, Zambia, Zimbabwe, Mozambique, DR Congo and South Africa, by means of analysis of external morphology, craniometry, enzymes, mitochondrial DNA sequences and karyology. Our results confirm that A. spinosissimus represents a complex of species with seemingly non-overlapping distribution ranges. The distribution range of A. spinosissimus appears to be restricted between the Zambesi and Limpopo Rivers, while the reinstated A. selousi (that includes A. transvaalensis) occurs further to the South (i.e. northern limit seemingly just north of the Limpopo River). The investigated populations north of the Zambezi River are morphologically and genetically distinct from A. spinosissimus and A. selousi. Based on this evidence, we described Acomys muzei sp. nov. and Acomys ngurui sp. nov., each one occurring separately along one side of the Eastern Arc Mountains. Finally, we lacked sufficient information to describe a third new species from the area north of the Zambesi River.References
Allen, G.M. (1939) A Checklist of African Mammals. Bulletin of the Museum of Comparative Zoology at Harvard College, 83, 1–763.
Barome, P.O., Monnerot, M. & Gautun. J.-C. (1998) Intrageneric phylogeny of Acomys (Rodentia, Muridae) using mitochondrial gene cytochrome b. Molecular Phylogenetics and Evolution, 9 (3), 560–566.
Barome, P.O., Monnerot, M. & Gautun. J.-C. (2000) Phylogeny of the genus Acomys (Rodentia, Muridae) based on the cytochrome b mitochondrial gene: implications on taxonomy and phylogeography. Mammalia, 64, 423–438.
Barome, P.O., Volobouev, V., Monnerot, M., Mfune, J.K., Chitaukali, W., Gautun, J.-C. & Denys, C. (2001) Phylogeny of Acomys spinosissimus (Rodentia, Muridae) from north Malawi and Tanzania: evidence from morphological and molecular analysis. Biological Journal of the Linnean Society, 73, 321–340.
Baskevich, M.I. & Lavrenchenko, L. (1995) On the morphology of spermatozoa in some African Murines (Rodentia, Muridae) The taxonomic and phylogenetic aspects. Journal of Systematic and Evolutionary Research, 33, 9–16.
Bibb, M.J., Van Etten, R. A, Wright, C.T., Walberg M.W. & Clayton, D.A. (1981) Sequence and gene organization of mouse mitochondrial DNA. Cell, 26, 167–180.
Chevret, P., Denys, C., Jaeger, J.-J., Michaux, J. & Catzeflis F.M. (1993) Molecular evidence that the spiny mouse (Acomys) is more closely related to gerbils (Gerbillinae) than to true mice (Murinae). Proceedings of the National Academy of Sciences USA, 90, 3433–3436.
Chevret, P. & Hänni, C. (1994) Systematics of the spiny mouse (Acomys: Muroidea): molecular and biochemical evidence. Israel Journal of Zoology, 40, 247–254.
Corbet, G.B. (1978) The Mammals of the Palaearctic Region: a Taxonomic Review. British Museum (Natural History), London, 314 pp.
Corti M., Castiglia, R., Colangelo, P., Capanna, E., Beolchini, F., Bekele, A., Oguge, N.O., Makundi, R.H., Sichilima, A.M., Leirs, H., Verheyen, W. & Verhagen, R. (2005) Cyto-taxonomy of rodent species from Ethiopia, Kenya, Tanzania and Zambia. Belgian Journal of Zoology, 135, Supplement, 97–216.
Denys, C., Gautun, J.C., Tranier, M. & Volobouev V. (1994) Evolution of the genus Acomys (Rodentia, Muridae) from dental and chromosomal patterns. Israel Journal of Zoology, 40, 215–246.
Denys, C. & Michaux, J. (1992a) La troisième molaire supérieure chez les Muridae d’Afrique tropicale et le cas des genres Acomys, Uranomys et Lophuromys. Bonner Zoologische Beiträge, 43, 355–365.
Denys, C., Michaux, J., Petter, F., Aguilar, J.P. & Jaeger, J.-J. (1992b) Molar morphology as a clue to the phylogenetic relationship of Acomys to the Murinae. Israel Journal of Zoology, 38, 253–262.
Dippenaar, N.J.J. & Rautenbach, I.L. (1986) Morphometrics and karyology of the southern African species of the genus Acomys I. Geoffroy Saint-Hilaire, 1838 (Rodentia: Muridae). Annals of the Transvaal Museum, 34, 129–183.
Ducroz, J.F., Volobouev, V. & Granjon, L. (1998) A molecular perspective on the systematics and evolution of the genus Arvicanthis (Rodentia, Muridae): Inferences from complete cytochrome b gene sequences. Molecular Phylogenetics and Evolution, 10, 104–117.
Felsenstein, J. (1991) PHYLIP 3.4. Department of Genetics. University of Washington. Seattle.
Hänni, C., Laudet, V., Barriel, V. & Catzeflis, F.M. (1995) Evolutionary relationships of Acomys and other Murids (Rodentia, Mammalia) based on complete 12S rRNA mitochondrial DNA sequences. Israel Journal of Zoology, 41, 131–146.
Hsu, T.C. & Patton, J.L. (1969) Bone marrow preparations for chromosome studies. In: K. Benirschke (Ed.), Comparative Mammalian Cytogenetics. New York, Springer–Verlag, pp. 454–460.
Janecek, L., Schlitter, D. A. & Rautenbach, I.L. (1991) A genic comparison of spiny mice, genus Acomys. Journal of Mammalogy, 72 (3), 542–552.
Matthey, R. (1954) Nouvelles données sur les formules chromosomiques des Muridae. Experientia, 10, 66–67.
Matthey, R. (1956) La formule chromosomique de quelques Murinae (Muridae, Rodentia, Mammalia). Archiv der Julius Klaus-Stiftung für Vererbungsforschung, Sozialanthropologie und Rassenhygiene, 31, 294–306.
Matthey, R. (1963) La formule chromosomique chez sept espèces et sous-espèces de Murinae africains. Mammalia, 27, 157–176.
Matthey, R. (1965a) Etudes de cytogénétique sur des Murinae africains appartenant aux genres Arvicanthis, Praomys, Acomys et Mastomys (Rodentia). Mammalia, 29, 228–249.
Matthey, R. (1965b) Le problème de détermination du sexe chez Acomys selousi de Winton. Cytogénétique du genre Acomys (Rodentia-Muridae). Revue Suisse de Zoologie, 72, 119–144.
Matthey, R. (1968) Cytogenetique et taxonomie du genre Acomys: A. percivali Dollman et A. wilsoni Thomas, espèces d’Abyssinie. Mammalia, 32, 621–627.
Musser, G.G. & Carleton, M.D. (1993) Family Muridae. In: Wilson, D.E., Reeder, D.M. (Eds.), Mammal Species of the World: A Taxonomic and Geographic Reference. 2nd edition, Smithsonian Institution Press, pp. 501–755.
Musser, G.G. & Carleton, M.D. (2005) Superfamily Muroidea. In: Wilson, D.E., Reeder, D.M. (Eds.), Mammal Species of the World: A Taxonomic and Geographic Reference. 3rd edition, Johns Hopkins University Press, Baltimore, pp. 894–1531.
Nei, M. (1972) Genetic distances between populations. American Naturalist, 106, 283–292.
Peters, W.C.H. (1852) Reise nach Mossambique. I. Säugethiere. Druck und Verlach, Berlin, 202
Petter, F. (1983) Eléments d’une révision des Acomys africains. Un sous-genre nouveau, Peracomys Petter et Roche, (1981) (Rongeurs: Murides). Annales Musée Royal de l’Afrique Centrale, Tervuren–Belgique, ser. 8 (Sciences Zoologiques), 237, 109–119.
Posada, D. (2008) jModelTest: Phylogenetic Model Averaging. Molecular Biology and Evolution, 25, 1253–1256.
Reynolds, J.B., Weir, B.S. & Cockerham, C.C. (1983) Estimation of the co-ancestry coefficient: basis for a short–term genetic distance. Genetics, 105, 767–779.
Ronquist, F., & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 19, 1572–1574.
Saitou, N & Nei, M. (1987) The Neighbor–joining method: A new Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406–442.
Sarich, V.M. (1985) Rodent Macromolecular Systematics. pp. 423–452, In: W.P. Luckett and J. -L. Hartenberger, (Eds.), Evolutionary Relationships among Rodents: A Multidisciplinary Analysis. Plenum Press, New York, 721 pp.
Setzer, H.W. (1975) Genus Acomys. Part 6.5. Pp. 1–2, In: J. Meester and H.W. Setzer, (Eds.), The Mammals of Africa: An Identification Manual. Smithsonian Institution Press, Washington, D.C., not paginated.
Sneath, P.H.A. & Sokal, R.R. (1973) Numerical Taxonomy. W.H. Freeman and Company, San Francisco. pp. 230–234.
Sokolov, V.E., Orlov, V.N., Baskevich, M.I., Bekele, A. & Mebrate, A. (1993) A karyological study of the spiny mouse Acomys Geoffroy 1838 (Rodentia, Muridae) along the Ethiopian Rift Valley. Tropical Zoology, 6, 227–235.
Sokolov, V.E., Orlov, V.N., Baskevich, M.I. & Mebrate, A. (1992) [Chromosomal sets of the spiny mice Acomys (Rodentia, Muridae) along the Ethiopian Rift Valley]. Zoologicheskii Zhurnal, 71, 116–124 (in Russian).
Suzuki, H., Tsuchiyia, K., & Takezaki, N. (2000) A molecular phylogenetic framework for the Ryuku endemic rodents Tokudaia osimensis and Diplothrix legata. Molecular Phylogenetics and Evolution, 15(1), 15–24.
StatSoft, Inc. (2003) STATISTICA version 6 (data analysis software system), www.statsoft.com.
Swofford, D.L. (2002) PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods) 4. 0 Beta. Smithsonian Institution, Washington D.C.
Swofford, D.L. & Selander, R.B. (1981) BIOSYS-1, A Computer Program For The Analysis Of Allelic Variation In Genetics. Department of Genetics and Development, University of Illinois, Urbana, 65 pp.
Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24,1596–1599.
Terryn, L., Wendelen, W., Leirs, H., Lenglet, G. & Verheyen, E. (2007). African Rodentia, http://projects.biodiversity.be/africanrodentia (09/09/2010).
Thomas, O. (1896) On the Mammals of Nyasaland: Fourth notice. Proceedings of the Zoological Society London, 788–798.
Van der Straeten, E. (1975) Lemniscomys bellieri, a new species of Muridae from the Ivory Coast (Mammalia, Muridae). Revue de Zoologie Africaine, 89, 906–908.
Van Rompaey, J., Verheyen, W. & Selens, M. (1984) Genetic differences between three species of pigmy-mice in Rwanda (Africa). Revue de Zoologie Africaine, 98, 886–894.
Verheyen, W., Colyn, M. & Hulselmans, J. (1996) Re-evaluation of the Lophuromys nudicaudus Heller, 1911 species complex with a description of a new species from Zaïre (Muridae–Rodentia). Bulletin van het Koninklijk Belgisch Instituut voor Natuurwetenschappen, Biologie, 66, 241–273.
Volobouev, V., Auffray, J.C., Debat, V., Denys C., Gautun, J.-C., & Tranier, M. (2007) Species delimitation in the Acomys dimidiatus - cahirinus complex inferred from chromosomal and morphological analyses. Biogical Journal of the Linnean Society, 91, 203–214.
Volobouev, V.T., Tranier, M. & Dutrilleux, B. (1991) Chromosome evolution in the genus Acomys: chromosome banding ana-lysis of Acomys cf. dimidiatus (Rodentia, Muridae). Bonner zoologische Beiträge, 4, 253–260.
Wright, S., (1978) Evolution and the genetics of populations. Vol. 4. Variability within and among natural populations. University of Chicago, Chicago. pp. 573.