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Structure of peptide solutions: A light scattering and numerical study

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Abstract.

We investigated the interactions between protein molecules in solution, in particular for low salt concentrations and thus strong electrostatic interactions where a treatment based on the second virial coefficient is not sufficient. Static and dynamic light scattering experiments on solutions containing the peptide human calcitonin (hCT) were combined with calculations based on the Ornstein-Zernike equation with the hypernetted chain (HNC) closure and computer simulations within the primitive electrolyte model. The simulation illustrates the distribution of proteins in solution and the formation of (transient) protein aggregates. It furthermore allows us to predict the physical stability of hCT solutions in dependence of ionic strength, pH and hCT concentration.

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Authors and Affiliations

  1. School of Physics, The University of Edinburgh, EH9 3JZ, Edinburgh, UK

    S. U. Egelhaaf

  2. Physics Department, University of Fribourg, 1700, Perolles, Fribourg, Switzerland

    V. Lobaskin & P. Schurtenberger

  3. Department of Applied BioSciences, ETH Zürich, 8057, Zürich, Switzerland

    H. H. Bauer & H. P. Merkle

Authors
  1. S. U. Egelhaaf
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  2. V. Lobaskin
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  5. P. Schurtenberger
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Correspondence to S. U. Egelhaaf.

Additional information

Received: 26 December 2003, Published online: 25 March 2004

PACS:

87.15.Nn Properties of solutions; aggregation and crystallization of macromolecules - 61.20.Qg Structure of associated liquids: electrolytes, molten salts, etc. - 87.14.Ee Proteins

V. Lobaskin: Present address: Max-Planck-Institut für Polymerforschung, 55128 Mainz, Germany

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Egelhaaf, S.U., Lobaskin, V., Bauer, H.H. et al. Structure of peptide solutions: A light scattering and numerical study. Eur. Phys. J. E 13, 153–164 (2004). https://doi.org/10.1140/epje/e2004-00051-2

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